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Merck
CN

850500P

Avanti

CHAPS

Avanti Research - A Croda Brand

别名:

3-[(3-氯胺丙基)二甲基氨基]-1-丙磺酸盐

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About This Item

经验公式(希尔记法):
C32H58N2O7S
CAS号:
分子量:
614.88
MDL编号:
UNSPSC代码:
12161902
NACRES:
NA.25

方案

>99% (TLC)

表单

powder

分子量

614.88 g/mol

包装

pkg of 1 × 1 g (850500P-1g)

制造商/商品名称

Avanti Research - A Croda Brand

技术

protein quantification: suitable

运输

dry ice

储存温度

−20°C

SMILES字符串

C[C@@]12C(CC[C@@H]2[C@@H](CCC(NCCC[N+](C)(CCCS(=O)([O-])=O)C)=O)C)C3[C@H](O)C[C@]4([H])C[C@H](O)CC[C@]4(C)C3C[C@@H]1O

InChI

1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1

InChI key

UMCMPZBLKLEWAF-BCTGSCMUSA-N

一般描述

CHAPS是用于膜生物化学的非变性两性离子去污剂。可用于溶解膜蛋白和破坏蛋白-蛋白各种相互作用。CHAPS的小胶束分子量(6,150;聚集数=10)和高临界胶束浓度(6-10 mM)使其可以通过透析从样品中除去。也适用于等电聚焦和二维电泳的蛋白增溶。CHAPS通常用于非变性(无尿素)IEF,并已显示出某些亚细胞制剂和植物蛋白具有出色的分离度。IEF凝胶通常使用2-4%(w/v)的浓度。

应用

CHAPS适用于制备双细胞,并研究脂质体溶解和转运蛋白复溶的过程。

包装

20 mL透明玻璃螺旋盖小瓶(850500P-1g)

法律信息

Avanti Research is a trademark of Avanti Polar Lipids, LLC

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Acute Tox. 4 Oral

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Liposome solubilization and membrane protein reconstitution using Chaps and Chapso
Cladera J, et al.
European Journal of Biochemistry, 243(3), 798-804 (1997)
Functional reconstitution of the nicotinic acetylcholine receptor by CHAPS dialysis depends on the concentrations of salt, lipid, and protein
Schuerholz T, et al.
Biochemistry, 31(21), 5067-5077 (1992)
Bilayer membrane modulation of membrane type 1 matrix metalloproteinase (MT1-MMP) structure and proteolytic activity
Cerofolini L, et al.
Scientific reports, 6, 29511-29511 (2016)
Stuart J Cordwell
Methods in molecular biology (Clifton, N.J.), 424, 139-146 (2008-03-29)
Reproducible techniques for the prefractionation of proteins prior to two-dimensional gel electrophoresis (2-DE) are essential for increasing the number of unique proteins that can be identified and assayed following biological experimentation. A simple and robust technique for separating highly soluble
Erik F Kot et al.
Langmuir : the ACS journal of surfaces and colloids, 34(11), 3426-3437 (2018-02-28)
Isotropic phospholipid bicelles are one of the most prospective membrane mimetics for the structural studies of membrane proteins in solution. Recent works provided an almost full set of data regarding the properties of isotropic bicelles; however, one major aspect of

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