产品名称
荧光转染试剂, Avanti Research™ - A Croda Brand, powder
assay
>99% (TLC)
form
powder
packaging
pkg of 1 × 10 mg (790326P-10mg)
technique(s)
transfection: suitable
lipid type
transfection
cationic lipids
shipped in
dry ice
storage temp.
−20°C
manufacturer/tradename
Avanti Research™ - A Croda Brand
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Biochem/physiol Actions
DOTAP:DOPE 与 0.1% NBD-DOPE 脂质体（LipNBD）用转铁蛋白重组，称为转铁蛋白脂质体-硝基苯并恶二唑（Tf-LipNBD），可用作探针。它在肿瘤细胞的体外磁共振成像（MRI） 中提供了改进的图像对比度。荧光转染试剂有助于了解纤维复合物膜结构和丝心蛋白与阳离子脂质的相互作用。N-（7-nitrobenz-2-oxa-1,3-diazol-4-yl）dioleoyl（NBD） 用于模型膜研究，可用于可视化膜域。
General description
荧光转染试剂具有比例为 1:1 的阳离子脂质 1,2-二油酰氧基丙基）-N,N,N-三甲基氯化铵（DOTAP）和中性脂质 DOPE（1,2-二油酰-sn-甘油-3-磷酸乙醇胺） w/w 以及 0.1 wt% N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)dioleoyl (NBD) DOPE。NBD-DOPE 是一种荧光探针，NBD 连接到 DOPE 的头部。
Packaging
5 mL透明玻璃密封安瓿（790326P-10mg）
Legal Information
Avanti Research is a trademark of Avanti Polar Lipids, LLC
Application
Transfection research
存储类别
11 - Combustible Solids
flash_point_f
No data available
flash_point_c
No data available
The influence of NBD fluorescent probe on model membranes containing POPC and DPPC
Weng CJ, et al.
Molecular Membrane Biology, 33(1-2), 23-28 (2016)
Efficiency data of intracellular recombinant protein delivery using cationic lipid coated silk fibroin particle
Kim WJ, et al.
Data in Brief, 13, 683-691 (2017)
Dual probe with fluorescent and magnetic properties for imaging solid tumor xenografts
Shan L, et al.
Molecular Imaging, 6(2), 7290-2007 (2007)
Woo-Jin Kim et al.
Biomaterials, 122, 154-162 (2017-01-26)
Directly delivering therapeutic proteins into cells has promise as an intervention without side effects for protein deficiencies caused by genetic defects. However, as negatively charged macromolecules, proteins require carriers for achieving cellular uptake and maintaining their activity in the cytoplasm.
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