form
powder
packaging
pkg of 1 × 1 g (100600P-1g), pkg of 1 × 100 mg (100600P-100mg), pkg of 5 × 100 mg (100600P-500mg)
manufacturer/tradename
Avanti Research™ - A Croda Brand
lipid type
lipid extracts
shipped in
dry ice
storage temp.
−20°C
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General description
该产品是37℃生长在Kornberg Minimal培养基中并在3/4对数生长期提取的大肠杆菌B（ATCC 11303）提取物。总大肠杆菌脂质提取物是各组织的氯仿：甲醇提取物。将该提取液用去离子水进行分配并浓缩氯仿相。极性脂质提取物是用丙酮沉淀并然后用乙醚进行提取的总脂质提取物。
Application
E. coli Extract Polar已被用于制备验证用脂质标准品。它还用于测定脂质的摩尔百分比。
E. coli Extract Polar已被用于：
- 脂质体制备，从而使用荧光膜染料劳丹进行光谱分析研究
- 多层膜囊泡的生成
- 用作串联质谱分析用脂质标准品
E. coli Extract Polar适用于：溶解在甲醇中制备脂质标准品制备小型单层囊泡制备脂质体（溶于Tris-盐酸 (HCl)）
Biochem/physiol Actions
E. coli Polar脂质提取物可用于重悬膜蛋白。磷脂酰乙醇胺是E. coli polar提取物主要的脂质成分。次要成分包括磷脂酰甘油（PG）和心磷脂。这些脂质主要来源于胞质膜小叶和外膜内小叶。E. coli polar脂质提取物可通过囊泡融合形成双层结构。
Packaging
5 mL透明玻璃密封安瓿瓶(100600P-100mg)
5 mL透明玻璃密封安瓿瓶(100600P-500mg)
60 mL棕色广口螺帽玻璃瓶(100600P-1g)
Legal Information
Avanti Research is a trademark of Avanti Polar Lipids, LLC
存储类别
11 - Combustible Solids
Using supported bilayers to study the spatiotemporal organization of membrane-bound proteins
Methods in Cell Biology, 128, 223-241 (2015)
Assessing Membrane Fluidity and Visualizing Fluid Membrane Domains in Bacteria Using Fluorescent Membrane Dyes
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Bio-protocol, 8(20) (2018)
Molecular model for the solubilization of membranes into nanodisks by styrene maleic acid copolymers
Scheidelaar S, et al.
Biophysical Journal, 108(2), 279-290 (2015)
Assessing membrane fluidity and visualizing fluid membrane domains in bacteria using fluorescent membrane dyes
Wenzel M, et al.
Bio-protocol (2018)
Miyer Patiño-Ruiz et al.
Scientific reports, 9(1), 15390-15390 (2019-10-30)
Bacterial NhaB Na+/H+ exchangers belonging to the Ion Transporter superfamily are poorly characterized in contrast to Na+/H+ exchangers of the Cation Proton Antiporter superfamily which have NhaA from Escherichia coli as a prominent member. For a more detailed understanding of
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