类型
for DNA synthesis
质量水平
产品线
Proligo Reagents
形式
liquid
储存条件
dry at room temperature
杂质
≤100 ppm water content (Karl Fischer)
颜色
clear
适用性
conforms to structure (GC, Formulation)
相容性
configured for (ÄKTA® and OligoPilot®)
储存温度
room temp
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应用
Cap A solution is used after the phosphoramidite reaction/coupling step. This solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.
制备说明
乙腈/N-甲基咪唑·8/2 (v/v)
其他说明
Cap A solution, containing acetonitrile (ACN) and N-methylimidazole (NMI), is mixed in situ with Cap B1 and Cap B2 solution during the capping (acetylation) reaction. Pyridine is applied as a mild base while NMI provides a powerful acylation catalyst.
法律信息
OligoPilot is a registered trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
警示用语：
Danger
危险分类
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Repr. 2 - Skin Corr. 1B
WGK
WGK 2
闪点(°F)
35.6 °F
闪点(°C)
2 °C
法规信息
危险化学品
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