产品名称
Protein delivery system, Human SCF, PODS®
biological source
(Spodoptera frugiperda (Sf9) cell culture)
form
(monodisperse crystals)
packaging
1 ea of 25 μg
technique(s)
: suitable using 3D bioprinting | cell culture
impurities
<0.06 EU/mL Endotoxin
color
colorless
storage temp.
2-8°C
Quality Level
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Application
PODS are composed of a bioactive cargo protein within a polyhedrin crystal lattice, with polyhedrin as the main protein. The 25 µg SKU size indicates the amount of bioactive cargo, typically 0.5% to 5% of the total protein. For instance, a 25 µg SKU at a 2% cargo loading contains 1.25 mg of total protein. PODS enable sustained release of cargo proteins when proteases degrade the polyhedrin scaffold. Growth factors are not bioavailable until released, and their concentration in media depends on the amount added and the release rate. In 10% serum, peak levels are usually reached within 24-48 hours, with about 20% of the cargo becoming soluble.
PODS often require lower concentrations than conventional growth factors, as they maintain effective levels. Pre-incubating with serum or proteases enhances availability, while conventional factors can be added for immediate effects. Optimal amounts should be determined empirically.
PODS often require lower concentrations than conventional growth factors, as they maintain effective levels. Pre-incubating with serum or proteases enhances availability, while conventional factors can be added for immediate effects. Optimal amounts should be determined empirically.
Features and Benefits
- Protein delivery system, PODS human SCF comes with molecular weight of 50 kDa, length 222 aa and endotoxin level <0.06 EU/ml. PODS co-crystals may be reconstituted at 200 million co-crystals/ml in sterile PBS. 20% glucose has a buoyant density closer to PODS co-crystals and can be useful for aliquoting.
- This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols. PODS co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8).
- PODS provides durability, stability and sustained release, addressing the limitations of conventional and enabling researchers to effortlessly control and manipulate cells in 2D and 3D culture as well as in-vivo.
General description
Protein delivery system, SCF, PODS contains the polyhedrin protein co-crystalized with Human SCF. Stem Cell Factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor c-Kit/CD117 and plays a critical role in the maintenance, survival, and differentiation of hematopoietic stem cells. Human SCF shows no activity on mouse cells, but mouse and rat SCF are active on human cells.
Legal Information
PODS is a registered trademark of Cell Guidance Systems Ltd
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Intravital Imaging Reveals Motility of Adult Hematopoietic Stem Cells in the Bone Marrow Niche
Samik Upadhaya et al.
Cell Stem Cell, 27, 336-345 (2020)
Barbara Jakimiuk et al.
Przeglad lekarski, 60(10), 663-667 (2004-04-01)
Stem cell factor (SCF) is a hematopoietic factor active in the early stages of hematopoiesis. Recognition of biological properties of SCF made a hope for its use in diagnosis and therapy of many diseases, especially neoplasmas. We present a characterization
Hematopoietic Stem Cell Factors: Their Functional Role in Self-Renewal and Clinical Aspects
Zoya Mann et al.
Frontiers in Cell and Developmental Biology, 10 (2022)
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