产品名称
Protein delivery system, Human CTGF, PODS®
biological source
(Spodoptera frugiperda (Sf9) cell culture)
form
(monodisperse crystals)
packaging
1 ea of 25 μg
technique(s)
: suitable using 3D bioprinting | cell culture
impurities
<0.06 EU/mL Endotoxin
color
colorless
storage temp.
2-8°C
Quality Level
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Application
PODS are composed of a bioactive cargo protein within a polyhedrin crystal lattice, with polyhedrin as the main protein. The 25 µg SKU size indicates the amount of bioactive cargo, typically 0.5% to 5% of the total protein. For instance, a 25 µg SKU at a 2% cargo loading contains 1.25 mg of total protein. PODS enable sustained release of cargo proteins when proteases degrade the polyhedrin scaffold. Growth factors are not bioavailable until released, and their concentration in media depends on the amount added and the release rate. In 10% serum, peak levels are usually reached within 24-48 hours, with about 20% of the cargo becoming soluble.
PODS often require lower concentrations than conventional growth factors, as they maintain effective levels. Pre-incubating with serum or proteases enhances availability, while conventional factors can be added for immediate effects. Optimal amounts should be determined empirically.
PODS often require lower concentrations than conventional growth factors, as they maintain effective levels. Pre-incubating with serum or proteases enhances availability, while conventional factors can be added for immediate effects. Optimal amounts should be determined empirically.
Features and Benefits
- Protein delivery system, PODS Human CTGF, comes with molecular weight of 16.4 kDa, length 143 aa, with endotoxin level of <0.06 EU/ml as measured by gel clot LAL assay. PODS co-crystals may be reconstituted at 200 million co-crystals/ml in sterile PBS. 20% glucose has a buoyant density closer to PODS co-crystals and can be useful for aliquoting.
- This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols. PODS co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8).
- PODS provides durability, stability AND sustained release, addressing the limitations of conventional and enabling researchers to effortlessly control and manipulate cells in 2D and 3D culture as well as in-vivo.
General description
Protein delivery system, Human CTGF, PODS, contains the polyhedrin protein co-crystalized with Human CTGF. Connective Tissue Growth Factor (CTGF) is a mitogen that is secreted by vascular endothelial cells in response to basic fibroblast growth factor (FGF-2) or vascular endothelial growth factor (VEGF). CTGF promotes cell growth, migration, adhesion, and survival of endothelial cells. CTGF is also important during osteogenesis, chondrogenesis, and skeletogenesis. CTGF has an insulin-like growth factor binding protein (IGFBP) domain, a thrombospondin type 1 repeat (TSR) domain, and a C-terminal cysteine knot motif.
Legal Information
PODS is a registered trademark of Cell Guidance Systems Ltd
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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Zihao Chen et al.
Frontiers in cell and developmental biology, 8, 593269-593269 (2020-11-17)
Connective tissue growth factor (CTGF) is a key signaling and regulatory molecule involved in different biological processes, such as cell proliferation, angiogenesis, and wound healing, as well as multiple pathologies, such as tumor development and tissue fibrosis. Although the underlying
Bongjun Kim et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 35(1), 155-166 (2019-09-11)
Metastasis to bone is a frequent occurrence in patients with breast and prostate cancers and inevitably threatens the patient's quality of life and survival. Identification of cancer-derived mediators of bone metastasis and osteolysis may lead to novel therapeutic strategies. In
Hyungjoo Kim et al.
Oncogene, 40(15), 2667-2681 (2021-03-12)
Connective tissue growth factor (CTGF), also known as CCN2, is a member of the CCN protein family of secreted proteins with roles in diverse biological processes. CTGF regulates biological functions such as cell proliferation, migration, adhesion, wound healing, and angiogenesis.
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