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一般描述
TLAM- Iδ1LVproS-13CH3 kit has 13CH3 isotopomer precursors and contains protocol instructions for creation of isotopically-labeled proteins.
应用
For detection of long-range nOes or for the NMR study of large proteins
TLAM- Iδ1LVproS-13CH3 is an optimized kit to produce proteins with 13CH3 isotopomer on regio and stereo-specific methyl groups of isoleucine, leucine and valine residues. This kit has been tested with protein isotopic labeling in E. coli. It is used in the detection of structurally meaningful long-range and intermolecular NOEs restraints in solution NMR spectroscopy.
包装
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.
Rime Kerfah et al.
Current opinion in structural biology, 32, 113-122 (2015-04-17)
Nuclear magnetic resonance (NMR) spectroscopy is a uniquely powerful tool for studying the structure, dynamics and interactions of biomolecules at atomic resolution. In the past 15 years, the development of new isotopic labeling strategies has opened the possibility of exploiting
Silke Wiesner et al.
Current opinion in structural biology, 35, 60-67 (2015-09-26)
Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that
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