推荐产品
等级
certified by the Biological Stain Commission
质量水平
形式
powder
组成
Dye content, 70%
颜色
blue to very dark blue
very dark green
mp
290 °C (dec.) (lit.)
溶解性
H2O: 1 mg/mL
λmax
633 nm
ε (消光系数)
≥11000 at 242-246 nm in H2O at -1
≥26000 at 287-291 nm in H2O
≥34000 at 620-634 μm in H2O
应用
diagnostic assay manufacturing
hematology
histology
储存温度
room temp
SMILES字符串
NC1=CC(SC(C2=N3)=CC(C=C2)=[N+](C)C)=C3C=C1.[Cl-]
InChI
1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
InChI key
NALREUIWICQLPS-UHFFFAOYSA-N
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应用
Azure A has been used to determine the amount of charged dextran sulfate in the nanoparticles for the study involving incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles.
生化/生理作用
The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye.
适用性
Certified for use in Lillie′s modified Nocht′s method and the NCCLS method for blood smears.
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
How Romanowsky stains work and why they remain valuable - including a proposed universal Romanowsky staining mechanism and a rational troubleshooting scheme.
Horobin RW
Biotechnic & Histochemistry, 86, 36-51 (2011)
Quantification and characterization of glycosaminoglycans at the nanogram level by a combined azure A-silver staining in agarose gels.
van de Lest CH, et al.
Analytical Biochemistry, 221, 356-361 (1994)
Heterochromatin in human male leukocytes.
Lima-de-Faria A and Reitalu J
The Journal of Cell Biology, 16.2, 315-322 (1963)
Paula Zaman et al.
International journal of nanomedicine, 11, 6149-6159 (2016-12-07)
Incorporation of proteins into dextran sulfate (DS)-chitosan (CS) nanoparticles (DSCS NPs) is commonly performed using entrapment procedures, in which protein molecules are mixed with DS and CS until particle formation occurs. As DS is an analog of heparin, the authors
Q Chen et al.
Analytical and bioanalytical chemistry, 382(7), 1513-1519 (2005-06-14)
The interaction between azur A (AA) and hyaluronic acid (HA) at AA concentrations from 3.430 x 10(-5) to 8.575 x 10(-5) M and sodium chloride concentrations from 0 to 0.01 M was investigated spectrophotometrically at 620 nm at temperatures from
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