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表单
powder
反应适用性
reagent type: cross-linking reagent
官能团
carbonyl
储存温度
2-8°C
SMILES字符串
N#CC#CC1=CC=C(C=O)C=C1
InChI
1S/C10H5NO/c11-7-1-2-9-3-5-10(8-12)6-4-9/h3-6,8H
InChI key
LYISHMGQKXLBHI-UHFFFAOYSA-N
应用
APN-Aldehyde is a bifunctional crosslinker for introduction of aldehyde into biomolecules. The coupling can be performed with high selectivity in biological medium using mild reaction conditions.
制备说明
Standard protein labeling procedure (cysteine labeling).
- Dissolve the protein in the appropriate buffer with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
- Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
- Incubate at room temperature for 2 hours.
- If necessary, purify the protein-aldehyde conjugate using size exclusion chromatography or ultrafiltration.
- The conjugate can be readily coupled with hydrazides/hydrazones/hydroxylamines using standard protocols.
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Sergii Kolodych et al.
Bioconjugate chemistry, 26(2), 197-200 (2015-01-24)
Amine-to-thiol coupling is the most common route for the preparation of antibody-drug conjugates (ADC). It is usually achieved by using heterobifunctional reagents possessing an activated ester at one end and a maleimide group at the other. However, maleimide-based conjugates were
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