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文件

760749

二苯并环辛基-PEG4-生物素结合物

Name: 二苯并环辛基-PEG4-生物素结合物
Brand: ALDRICH

for Copper-free Click Chemistry

别名:

DBCO-PEG4-生物素

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About This Item

经验公式（希尔记法）:

C₃₉H₅₁N₅O₈S

CAS号:

1255942-07-4

分子量:

749.92

MDL编号:

MFCD22572661

UNSPSC代码:

12352003

PubChem化学物质编号:

329766893

NACRES:

NA.22

质量水平

100

形式

solid

反应适用性

reaction type: click chemistry

储存温度

−20°C

SMILES字符串

O=C(CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@H]12)NCCOCCOCCOCCOCCC(=O)NCCC(=O)N3Cc4ccccc4C#Cc5ccccc35

InChI

1S/C39H51N5O8S/c45-35(12-6-5-11-34-38-32(28-53-34)42-39(48)43-38)41-18-20-50-22-24-52-26-25-51-23-21-49-19-16-36(46)40-17-15-37(47)44-27-31-9-2-1-7-29(31)13-14-30-8-3-4-10-33(30)44/h1-4,7-10,32,34,38H,5-6,11-12,15-28H2,(H,40,46)(H,41,45)(H2,42,43,48)/t32-,34-,38-/m0/s1

InChI key

LNHSQAOQVNHUGL-QRBHCBQLSA-N

一般描述

这种氮杂二苯并环辛-生物素衍生物是一种通用的生物素化试剂，用于标记含有叠氮化物的分子或化合物。环辛烯在促进无铜叠氮炔环加成反应中是有用的。这种二苯并环辛炔将与叠氮官能化化合物或生物分子反应，而不需要 Cu (I) 催化剂产生稳定的三唑键。后续的标记分子可以使用链霉亲和素或亲和素亲和试剂进行纯化。PEG 间隔基使整体化合物的亲水性更强，并允许与叠氮化物的反应站点和生物素检测基团之间的距离更大。

应用

采用 Synple 自动合成平台素（SYNPLE-SC002）自动标记生物素

特点和优势

更高的温度和过多的生物素试剂会导致更有效的标记。典型反应时间长达 4 小时，但如果浓度较低或观察到低标记，则应使用更长的孵育时间。当首先溶解在水溶性有机溶剂中，然后根据需要在水性反应缓冲液中稀释时，产品最有效。DBCO-PEG4-生物素可溶于高达 0.35 mM 的水性缓冲液。

其他说明

2024年CiteAb奖，授予在帕金森研究方面取得成功的供应商

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

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Robust fluorescent detection of protein fatty-acylation with chemical reporters.

Guillaume Charron et al.

Journal of the American Chemical Society, 131(13), 4967-4975 (2009-03-14)

Fatty-acylation of proteins in eukaryotes is associated with many fundamental cellular processes but has been challenging to study due to limited tools for rapid and robust detection of protein fatty-acylation in cells. The development of azido-fatty acids enabled the nonradioactive

Ning, X., et al.

Angewandte Chemie (International Edition in English), 47, 2253-2255 null

Identification of protein targets of 4-hydroxynonenal using click chemistry for ex vivo biotinylation of azido and alkynyl derivatives.

Andrew Vila et al.

Chemical research in toxicology, 21(2), 432-444 (2008-02-01)

Polyunsaturated fatty acids (PUFA) are primary targets of free radical damage during oxidative stress. Diffusible electrophilic alpha,beta-unsaturated aldehydes, such as 4-hydroxynonenal (HNE), have been shown to modify proteins that mediate cell signaling (e.g., IKK and Keap1) and alter gene expression

Labeling, detection and identification of newly synthesized proteomes with bioorthogonal non-canonical amino-acid tagging.

Daniela C Dieterich et al.

Nature protocols, 2(3), 532-540 (2007-04-05)

A major aim of proteomics is the identification of proteins in a given proteome at a given metabolic state. This protocol describes the step-by-step labeling, purification and detection of newly synthesized proteins in mammalian cells using the non-canonical amino acid

A comparative study of bioorthogonal reactions with azides.

Nicholas J Agard et al.

ACS chemical biology, 1(10), 644-648 (2006-12-19)

Detection of metabolites and post-translational modifications can be achieved using the azide as a bioorthogonal chemical reporter. Once introduced into target biomolecules, either metabolically or through chemical modification, the azide can be tagged with probes using one of three highly

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