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形式
liquid
浓度
3.0 M in methylene chloride
溶解性
alcohol: freely soluble
diethyl ether: freely soluble
water: freely soluble
密度
1.443 g/mL at 25 °C
SMILES字符串
BrC#N
InChI
1S/CBrN/c2-1-3
InChI key
ATDGTVJJHBUTRL-UHFFFAOYSA-N
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一般描述
溴化氰溶液仅在N-取代吗啉存在下诱导模板引导的寡核苷酸缩合。研究了N-取代吗啉缓冲液中溴化氰激活磷酸单酯基团的机理。
应用
溴化氰（CNBr）溶液可用于溴化氰法将胰蛋白酶共价固定在聚（2-羟乙基甲基丙烯酸酯）/聚苯乙烯复合微球上。它可用于制备CNBr活化的二醇二氧化硅，可用作HPLC中的二氧化硅载体。
警示用语：
Danger
危险分类
Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Acute Tox. 3 Dermal - Carc. 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3
靶器官
Central nervous system
补充剂危害
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
法规信息
危险化学品
Luis A Jurado et al.
Journal of chromatography. A, 971(1-2), 95-104 (2002-09-28)
To obtain silica supports for high-performance affinity chromatography, a method of preparing CNBr-activated diol-silica under anhydrous conditions was developed. Activation of the silane-derived hydroxyls with cyanogen bromide and triethylamine was optimized and demonstrated to efficiently couple several amino ligands (tryptophan
Z A Shabarova et al.
Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life, 27(5-6), 555-566 (2001-09-07)
Cyanogen bromide has been found to induce the template-guided condensation of oligonucleotides only in the presence of N-substituted morpholines. Based on 31P, 1H and 13C NMR spectroscopy data, the mechanism of the phosphomonoester group activation by cyanogen bromide in N-substituted
Covalent immobilization of trypsin onto poly (2-hydroxyethyl methacrylate)/polystyrene composite microspheres by cyanogen bromide method and its enzymatic activity.
Okubo M, et al.
Colloid and Polymer Science, 265(11), 957-964 (1987)
Arthur T Kopylov et al.
Proteomics, 13(5), 727-742 (2013-01-03)
In this paper, we present a method for the determination of low- and ultralow copy-number proteins in biomaterials based on a combination of concentrating the protein from the sample onto cyanogen bromide-activated Sepharose 4B (via nonspecific binding of free amino
P P Moerman et al.
Journal of proteomics, 73(8), 1454-1460 (2010-02-16)
We present a novel approach to perform C-terminal sequence analysis by discriminating the C-terminal peptide in a mass spectral analysis of a CNBr digest. During CNBr cleavage, all Met-Xxx peptide bonds are cleaved and the generated internal peptides all end
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