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341738

Sigma-Aldrich

磺酰罗丹明 B，酸式

laser grade, Dye content 95 %

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About This Item

经验公式（希尔记法）:
C27H30N2O7S2
CAS号:
2609-88-3
分子量:
558.67
EC 号:
220-025-2
MDL编号:
MFCD00067690
UNSPSC代码:
12352103
PubChem化学物质编号:
57649121
NACRES:
NA.23

等级

laser grade

质量水平

200

表单

solid

组成

Dye content, 95%

mp

>300 °C (lit.)

λmax

558 nm

SMILES字符串

CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S(O)(=O)=O)=[N+](/CC)CC)c1

InChI

1S/C27H30N2O7S2/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35)

InChI key

IOOMXAQUNPWDLL-UHFFFAOYSA-N

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相关类别

一般描述

磺酰罗丹明 B (SRB) 是一种荧光蛋白染料。 据报告，染料在温和的碱性条件下与三氯乙酸固定细胞的氨基酸残基结合，并在温和的酸性条件下将其自身提取到溶液中。

应用

SRB 对其对活细胞反应性的 pH 敏感性使其可用于药物诱导的细胞毒性和细胞增殖。SRB 实验用于监测活细胞的酶活性。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCL1013
20223EA
05605DC
04920EQ
11917BU

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Wieland Voigt
Methods in molecular medicine, 110, 39-48 (2005-05-20)
The sulforhodamine B (SRB) assay was developed by Skehan and colleagues to measure drug-induced cytotoxicity and cell proliferation for large-scale drug-screening applications. Its principle is based on the ability of the protein dye sulforhodamine B to bind electrostatically and pH
Efficacy of 5-FU or Oxaliplatin Monotherapy over Combination Therapy in Colorectal Cancer.
Toloudi M, et al.
Journal of Cancer Therapy, 6(4), 345-345 (2015)
Minerva Nájera-Martínez et al.
Inhalation toxicology, 24(11), 762-773 (2012-09-08)
Halomethanes (HMs) can be formed during the chlorination process to obtain drinking water. In liver cells, HMs had been shown to be mutagenic and carcinogenic; however, their bioactivation by CYP 2E1 and GSTT1 is required. Although inhalation is the most
E E Gurdal et al.
Drug research, 63(3), 121-128 (2013-03-01)
This study presents the synthesis of nineteen 1-(substitutedbenzoyl)-4-benzhydrylpiperazine and 1-[(substitutedphenyl)sulfonyl]-4-benzhydrylpiperazine derivatives. In vitro cytotoxic activities of the compounds were screened against hepatocellular (HUH-7), breast (MCF-7) and colorectal (HCT-116) cancer cell lines by sulphorhodamine B assay. Among the test compounds, benzamide
Lindsay N Sanford et al.
Analytical biochemistry, 434(1), 26-33 (2012-11-13)
Accurate control of the sample temperature during thermal cycling is critical for successful polymerase chain reaction (PCR). Direct sensor contact with the reaction is problematic, forcing measurements external to the sample and compromising accuracy during rapid temperature transitions. The widespread
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