230162
磺酰罗丹明B
Dye content 75 %
别名:
磺酰罗丹明B 单钠盐, 酸性红52
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关于此项目
经验公式（希尔记法）:
C27H29N2NaO7S2
化学文摘社编号:
分子量:
580.65
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
Colour Index Number:
45100
EC Number:
222-529-8
MDL number:
Beilstein/REAXYS Number:
3886008
产品名称
磺酰罗丹明B, Dye content 75 %
InChI key
SXQCTESRRZBPHJ-UHFFFAOYSA-M
InChI
1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1
SMILES string
[Na+].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](\CC)CC)c1
assay
≥73% (HPLC)
form
powder
composition
Dye content, 75%
technique(s)
microbe id | staining: suitable
color
brown to very dark brown
green to very dark green
solubility
methanol: 1 mg/mL
λmax
554 nm
ε (extinction coefficient)
≥85000 at 554-560 nm in 0.1 M NaOH in methanol at 0.005 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
Application
磺酰罗丹明B已被用作MCF-7（人乳腺癌细胞系）细胞增殖实验的染料。
General description
磺酰罗丹明B（SRB）是一种亮粉色氨基甲氧杂蒽类染料。磺酰罗丹明B（SRB）测定被广泛用于 体外 细胞毒性筛选。SRB以化学计量方式与细胞的蛋白质成分结合。因此，从染色细胞中提取的染料量与细胞质量成正比。SRB还可用于细胞密度测定。
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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