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Merck
CN

198080

Sigma-Aldrich

甲基绿

zinc chloride salt, certified by the Biological Stain Commission, Dye content 85 %

别名:

乙基绿

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About This Item

线性分子式:
C27H35BrClN3 · ZnCl2
CAS号:
分子量:
653.24
颜色索引号:
42590
Beilstein:
3914106
EC 号:
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.47

等级

certified by the Biological Stain Commission

质量水平

形式

powder

组成

Dye content, 85%

pH值(酸碱度)

0.2-1.8, yellow to blue

mp

>300 °C (lit.)

溶解性

H2O: 1 mg/mL

λmax

423 nm (2nd)
629 nm

ε (消光系数)

≥11100 at 417-425 in H2O at 0.005 g/L
≥16000 at 311-319 in H2O at 0.005 g/L
≥60000 at 626-634 in H2O at 0.005 g/L

应用

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES字符串

[Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C

InChI

1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4

InChI key

RRKSQWOQZFIQKW-UHFFFAOYSA-J

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一般描述

Methyl Green is a basic dicationic dye and is composed of triphenylmethane. It is suitable for the detection of DNA and RNA. It acts as a photochromophore and used to activate gelatinous films. At a very high pH, methyl green forms an alkaline solution and gets converted to a colorless carbinol base. In the presence of hydroxide anion, methyl green produces crystal violet dye and ethanol.

适用性

Suitable for use as a DNA stain.

警示用语:

Danger

危险声明

危险分类

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3

靶器官

Respiratory system

WGK

WGK 3

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves


分析证书(COA)

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The problem of a standardized technic for the methyl-green-pyronin stain.
E B TAFT
Stain technology, 26(3), 205-212 (1951-07-01)
R.D. Lillie Ed.
Conn?s Biological Stains, 282-282 (1977)
Mechanistic studies of the photocatalytic degradation of methyl green: an investigation of products of the decomposition processes
Chen CC and Lu CS
Environmental Science & Technology, 41(12), 4389-4396 (2007)
Histopathologic Technic and Practical Histochemistry, 183-183 (1954)
Photodegradation of methyl green using visible irradiation in ZnO suspensions: determination of the reaction pathway and identification of intermediates by a high-performance liquid chromatography--photodiode array-electrospray ionization-mass spectrometry method
Mai FD, et al.
Journal of Chromatography A, 1189(1-2), 355-365 (2008)

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