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Merck
CN

W1754

水

PCR Reagent, suitable for PCR

别名:

H2O

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关于此项目

线性分子式:
H2O
化学文摘社编号:
7732-18-5
分子量:
18.02
Beilstein:
2050024
EC 号:
231-791-2
MDL编号:
MFCD00011332
UNSPSC代码:
12191602
PubChem化学物质编号:
24900779
NACRES:
NA.25

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产品名称

水, PCR Reagent

蒸汽密度

<1 (vs air)

质量水平

200

蒸汽压

3 mmHg

无菌性

sterile-filtered

表单

liquid

包装

vial of 1.5 mL

技术

PCR: suitable

折射率

n20/D 1.34 (lit.)

pH值（酸碱度）

5-7

沸点

100 °C (lit.)

mp

0 °C (lit.)

密度

1.000 g/mL at 3.98 °C (lit.)

异质活性

DNase, none detected
RNase, none detected

SMILES字符串

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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相关类别

一般描述

对 PCR 级水进行无菌过滤。它不含核酸外切酶（dna 酶、rna 酶）和核酸内切酶（切口酶），也不含核酸污染。

应用

水已用于：

  • 作为反应混合物的组分和微流控RT-qPCR的稀释剂
  • 作为反应混合物的组分，以扩增真菌（杂色云芝）DNA的产物
  • 作为稀释剂和反应混合物的组分，以扩增cDNA
  • 水已用于在聚合酶链式反应 (PCR) 中补充样品的最终体积
适用于聚合酶链式反应 (PCR)

其他说明

便于将水产品规格与 水规格表进行比较。

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000423209
0000423207
0000423208
0000423207
0000423208

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N Wellinghausen et al.
Applied and environmental microbiology, 67(9), 3985-3993 (2001-08-30)
Contamination of hospital water systems with legionellae is a well-known cause of nosocomial legionellosis. We describe a new real-time LightCycler PCR assay for quantitative determination of legionellae in potable water samples. Primers that amplify both a 386-bp fragment of the
Incidence and survival of non-O157 verocytotoxigenic Escherichia coli in soil
Bolton DJ, et al.
Journal of Applied Microbiology, 111(2), 484-490 (2011)
Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases.
Matthew J Ellington et al.
The Journal of antimicrobial chemotherapy, 59(2), 321-322 (2006-12-23)
Francine Marciano-Cabral et al.
Applied and environmental microbiology, 69(10), 5864-5869 (2003-10-09)
The free-living amoeboflagellate Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis (PAM), a rapidly fatal disease of the central nervous system. In the United States, the disease is generally acquired while swimming and diving in freshwater lakes and
J Loeffler et al.
Journal of clinical microbiology, 37(4), 1200-1202 (1999-03-13)
Successful in vitro amplification of fungal DNA in clinical specimens has been reported recently. In a collaboration among five European centers, the frequency and risk of contamination due to airborne spore inoculation or carryover contamination in fungal PCR were analyzed.
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实验方案

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

Antibody-Enzyme Mediated Hot Start PCR Protocol

JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.

Hot Start dNTP Protocol

Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.

Amplification of Genomic DNA using REDTaq® DNA Polymerase

Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.

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