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B9879

Sigma-Aldrich

N，N-双（2-羟乙基）-2-氨基乙磺酸

≥99.0% (titration)

别名:

2-(双(2-羟乙基)氨基)乙磺酸, n,n-双[2-羟乙基]-2-氨基乙磺酸, 2-(二乙醇氨基)乙磺酸, N,N-双(2-羟乙基)-2-氨基乙磺酸

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5 G
¥870.17
25 G
¥3,624.03
100 G
¥12,155.56

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5 G
¥870.17
25 G
¥3,624.03
100 G
¥12,155.56

About This Item

经验公式（希尔记法）:
C6H15NO5S
CAS号:
10191-18-1
分子量:
213.25
Beilstein:
1781572
EC 号:
233-465-5
MDL编号:
MFCD00007533
UNSPSC代码:
12161700
PubChem化学物质编号:
24892162
NACRES:
NA.25

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方案

≥99.0% (titration)

表单

crystalline

储存条件

dry at room temperature

颜色

white

pH值（酸碱度）

2.5-5.0 (25 °C, 213.3 g/L)

有效pH范围

6.4-7.8

pKa (25 °C)

7.1

溶解性

water: 0.5 g/mL, clear, colorless

密度

1.30 g/cm3 at 20—25 °C
1.35 g/cm3 at 20—25 °C

应用

clinical research
diagnostic assay manufacturing
general analytical
life science and biopharma

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此商品
N5538SAB4300426N4142
抗硝基酪氨酸 兔抗 affinity isolated antibody, buffered aqueous solution

N0409

抗硝基酪氨酸 兔抗

单克隆抗-3-硝基酪氨酸 小鼠抗 clone 18G4, purified immunoglobulin, buffered aqueous glycerol solution

N5538

单克隆抗-3-硝基酪氨酸 小鼠抗

Anti-NOS1 (Ab-852) antibody produced in rabbit affinity isolated antibody

SAB4300426

Anti-NOS1 (Ab-852) antibody produced in rabbit

抗-神经丝200 兔抗 IgG fraction of antiserum, buffered aqueous solution

N4142

抗-神经丝200 兔抗

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

Quality Level

200

Quality Level

-

Quality Level

100

Quality Level

200

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

Gene Information

human ... NOS1(4842)

Gene Information

human ... NOS1(4842)

Gene Information

human ... NOS1(4842)

Gene Information

human ... NEFH(4744)
mouse ... Nefh(380684)
rat ... Nefh(24587)

technique(s)

immunohistochemistry (frozen sections): 1:1000 using methacarn-fixed, frozen sections of human Alzheimer′s Disease brain, indirect immunofluorescence: 1:1,000 using A549 cells metabolically labeled with 3-nitrotyrosine, western blot: 1:1,000 using nitrated-BSA, indirect ELISA: 1:1,000 using nitrated-BSA, microarray: suitable

technique(s)

western blot: 1:1,000 using mouse and rat brain

technique(s)

indirect immunofluorescence: 1:100-1:200

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80 using animal cerebellum sections, microarray: suitable, western blot: suitable

一般描述

N,N-双(2-羟乙基)-2-氨基乙磺酸，即BES，是一种两性离子缓冲液，是生物化学研究中具有重要价值的二级标准物质。BES是一种含磺酸的交联剂，可在磺化聚酰亚胺膜中诱导磺化聚酰亚胺链之间的交联。BES缓冲液的有效pH范围是6.4-7.8。它是20世纪60年代开发的“良好”缓冲液之一，可在6.15-8.35的pH范围内产生缓冲作用，广泛用于生化研究。BES缓冲液适用于需要形成磷酸钙-DNA复合物的体系（吸光度： < 在260 nm处为0.05，100 mM）。另外，BES缓冲液可用于分子生物学、生物缓冲液、分子生物学试剂和两性离子化合物的研究。

应用

另外，BES在转染研究中也有应用，包括转染试剂的制备[1]和与神经黑色素受体相关的信号传导分析[2]。
在人黑素瘤细胞的结合测定期间，BES可用作改良Eagle′培养基中的结合缓冲液。[3]它可以用作生物缓冲剂来研究异金属CuII/Li 3D配位聚合物的水性介质自组装过程。[4]

特点和优势

  • 适用于细胞培养基和在生化和分子生物学研究中用作缓冲溶液
  • pKa为7.1（25 °C），可在pH 2.5-5.0（25 °C，213.3 g/L）内有效缓冲
  • 经测试，确认重金属含量很低，确保适用于各种应用

其他说明

如需了解生化试剂系列的更多信息，请填写此表。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    0000438296
    0000437402
    0000430304
    0000425085
    0000425084

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    Water sorption, proton conduction, and methanol permeation properties of sulfonated polyimide membranes cross-linked with N, N-bis (2-hydroxyethyl)-2-aminoethanesulfonic acid (BES).
    Lee CH, et al.
    Macromolecules, 39(2), 755-764 (2006)
    Marina V Kirillova et al.
    Inorganic chemistry, 51(9), 5224-5234 (2012-04-10)
    The facile aqueous medium reactions of copper(II) nitrate with BES biobuffer [(HOCH(2)CH(2))(2)N(CH(2)CH(2)SO(3)H), hereinafter referred as H(3)bes] in the presence of various benzenecarboxylic acids [benzoic (Hba), 3-hydroxybenzoic (Hhba), and 3,5-dihydroxybenzoic (Hdhba) acid] and lithium hydroxide gave rise to the self-assembly generation
    Second-stage dissociation of N,N-bis (2-hydroxyethyl)-2-aminoethanesulfonic acid (?bes?) in water and in 50 mass per cent methanol+ water from 278.15 to 328.15 K.
    Roy RN, et al.
    The Journal of Chemical Thermodynamics, 9(4), 325-332 (1977)
    Ya-Xiong Tao et al.
    Methods in enzymology, 484, 267-279 (2010-11-03)
    The two neural melanocortin receptors (MCRs), melanocortin-3 and -4 receptors (MC3R and MC4R), are G protein-coupled receptors expressed primarily in the brain that regulate different aspects of energy homeostasis. The MCRs are unique in having endogenous antagonists, agouti and agouti-related
    Logan Y Brown et al.
    STAR protocols, 1(3), 100152-100152 (2020-12-31)
    Lentiviral vectors are an ideal gene-delivery system for large gene-editing tools, such as the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system, due to their high packaging capacity and broad tropism. Here, we present a calcium phosphate-based protocol for lentiviral
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