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B9754

Sigma-Aldrich

2-二(2-羟乙基)氨基-2-羟甲基-1,3-丙二醇

≥98.0% (titration)

别名:

2-二(2-羟乙基)氨基-2-羟甲基-1,3-丙二醇, 2-双(羟基甲基)-2,2′,2″-次氮基三乙醇, 双(2-羟乙基)氨基-三(羟甲基)甲烷

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选择尺寸

100 μG
CN¥4,472.02

CN¥4,472.02

国内现货，预计发货时间April 27, 2025详情

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100 μG
CN¥4,472.02

About This Item

经验公式（希尔记法）:
C8H19NO5
CAS号:
6976-37-0
分子量:
209.24
Beilstein:
2205275
EC 号:
230-237-7
MDL编号:
MFCD00002853
UNSPSC代码:
12161700
PubChem化学物质编号:
24892155
NACRES:
NA.25

CN¥4,472.02

国内现货，预计发货时间April 27, 2025详情

获取大包装报价

质量水平

400

方案

≥98.0% (titration)

表单

crystalline powder

储存条件

dry at room temperature

技术

UV/Vis spectroscopy: suitable
electrophoresis: suitable

颜色

white

有效pH范围

5.8-7.2

pKa (25 °C)

6.5

溶解性

H2O: 500 mg/mL, clear, colorless to very faintly yellow

适用性

suitable for Western blot
suitable for column chromatography of proteins

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此商品
HPA012558HPA012828HPA008273
抗 MAP2 兔抗 ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

M3696

抗 MAP2 兔抗

Anti-MAP2 antibody produced in rabbit Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

HPA012558

Anti-MAP2 antibody produced in rabbit

抗 MAP2 兔抗 Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

HPA012828

抗 MAP2 兔抗

抗 MAP2 兔抗 affinity isolated antibody, buffered aqueous glycerol solution

HPA008273

抗 MAP2 兔抗

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

mol wt

antigen ~280 kDa, antigen ~72 kDa

mol wt

-

mol wt

-

mol wt

-

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

一般描述

Bis-tris 甲烷，也称为 BIS-TRIS 或 BTM，是一种广泛应用于生物化学和生物研究的两性离子缓冲剂。它在 pH 值范围为 5.8 至 7.2 的水溶液中有效发挥作用，并且其解离常数变化最小。此外，它可以用作剧毒缓冲剂二甲胂酸盐的替代品，并且还因其与不同金属形成络合物的能力而受到认可。

应用

Bis-Tris已被使用：
  • 作为电泳缓冲液[1]
  • 作为蛋白质印迹转移缓冲液的组分[2]

特点和优势

  • 适用于生物和生化研究
  • 在 pH 5.8-7.2 (25 °C) 范围内有效缓冲，pKa 为 6.5 (25 °C)

其他说明

如需了解生化试剂系列的更多信息，请填写此表。

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H319

危险分类

Eye Irrit. 2

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    0000438295
    0000438295
    0000355113
    0000355113
    0000433721

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    如果您需要特殊版本，可通过批号或批次号查找具体证书。

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    在文件库中查找您最近购买产品的文档。

    访问文档库

    Raman Kumar et al.
    STAR protocols, 3(4), 101693-101693 (2022-09-20)
    Immunoprecipitation (IP) of endogenously expressed proteins is one of the most biologically relevant techniques to identify protein-protein interactions. We describe an adaptable IP protocol reliant on a specific antibody to the target protein. We detail a quantitative proteomics workflow for
    Alastair W Skeffington et al.
    Plant physiology, 165(2), 866-879 (2014-05-02)
    The first step on the pathway of starch degradation in Arabidopsis (Arabidopsis thaliana) leaves at night is the phosphorylation of starch polymers, catalyzed by glucan, water dikinase (GWD). It has been suggested that GWD is important for the control of
    Zahra Sabouri et al.
    Proceedings of the National Academy of Sciences of the United States of America, 111(25), E2567-E2575 (2014-05-14)
    The best-understood mechanisms for achieving antibody self/non-self discrimination discard self-reactive antibodies before they can be tested for binding microbial antigens, potentially creating holes in the repertoire. Here we provide evidence for a complementary mechanism: retaining autoantibodies in the repertoire displayed
    Elizabeth M MacDonald et al.
    Disease models & mechanisms, 7(4), 471-481 (2014-02-08)
    The purpose of our study was to compare two acquired muscle atrophies and the use of myostatin inhibition for their treatment. Myostatin naturally inhibits skeletal muscle growth by binding to ActRIIB, a receptor on the cell surface of myofibers. Because
    Lesley Cheng et al.
    Kidney international, 86(2), 433-444 (2013-12-20)
    Micro RNAs (miRNAs) have been shown to circulate in biological fluids and are enclosed in vesicles such as exosomes; they are present in urine and represent a noninvasive methodology to detect biomarkers for diagnostic testing. The low abundance of RNA
    查看所有相关文献

    实验方案

    Enzymatic Activity of Glucose-6-Phosphatase [EC 3.1.3.9]

    To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.

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