¥3,748.00
国内现货，预计发货时间2025年4月22日详情
推荐产品
方案
≥85% (HPLC)
质量水平
表单
solution
分子量
Mr 1090.7
包装
pkg of 125 μL (11558706910 [1 mM])
pkg of 5 × 125 μL (11570013910 [5x1 mM])
pkg of 25 μL (11093088910 [1 mM])
制造商/商品名称
Roche
λmax
222 and 292 nm
22430 and 8780
储存温度
−20°C
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此商品 | DIUTPRO | 11363905910 | DIGUTPRO |
|---|---|---|---|
| manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche |
| form solution | form solution | form solution | form solution |
| assay ≥85% (HPLC) | assay 85% (HPLC) | assay 85% (HPLC) | assay ≥85% (HPLC) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| packaging pkg of 125 μL (11558706910 [1 mM]), pkg of 25 μL (11093088910 [1 mM]), pkg of 5 × 125 μL (11570013910 [5x1 mM]) | packaging pkg of 125 μL (11573179910 [1 mM]]]), pkg of 25 μL (11573152910 [1 mM]) | packaging pkg of 25 μL (25 nmol; 1mM) | packaging pkg of 25 μL (11209256910 [10 mM]), pkg of 57 μL (03359247910 [3.5 mM]) |
一般描述
异羟基洋地黄毒苷-11-dUTP对碱性稳定，存在于1 mM四锂盐溶液中。
应用
生化/生理作用
异羟基洋地黄毒苷(DIG)-11-三磷酸脱氧尿苷(dUTP)在随机引物 DNA标记反应中或在切口翻译中以35％DIG-11-dUTP和65％dTTP的比例替换三磷酸脱氧胸苷(dTTP)。DIG-11-dUTP 还可以在聚合酶链式反应(PCR)期间（例如，使用Taq DNA聚合酶），3′-加尾（具有末端转移酶）和cDNA合成（例如，使用转录物或 M-MuLV逆转录酶）在标记中替换dTTP。它是DNA聚合酶、Taq DNA聚合酶、末端转移酶和逆转录酶的理想底物。
特点和优势
地高辛通过耐碱性醚键与脱氧尿苷三磷酸结合。
耐碱性：在+ 15至+ 25℃条件下，该制剂在0.1-0.5M NaOH 存在时稳定。
耐碱性：在+ 15至+ 25℃条件下，该制剂在0.1-0.5M NaOH 存在时稳定。
质量
典型分析：至少 85％ DIG-11-dUTP（HPLC，面积％）。
通过 RPL 测试的功能。
通过 RPL 测试的功能。
制备说明
工作溶液：我们建议制备含有dATP、dCTP、dGTP、dTTP（各 10mM）的脱氧核苷酸混合物；（例如，为了制备100μl 核苷酸混合物，将 10μl dATP、dCTP、dGTP、dTTP（各）加至 60μl 水，PCR 级）。
储存条件（工作溶液）：在15至25℃条件下，4周内可能发生约 5％的分解。
储存条件（工作溶液）：在15至25℃条件下，4周内可能发生约 5％的分解。
其他说明
仅用于生命科学研究。不可用于诊断。
储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
does not flash
闪点(°C)
does not flash
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