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Key Documents

G005861

HPLC Analysis of Fat-Soluble Vitamins on Ascentis® Express C18, Methanol Mobile Phase

application for HPLC

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100 ML
CN¥1,167.96
250 ML
CN¥2,915.19
4 X 100 ML
CN¥5,649.38
800 ML
CN¥8,418.61
1 L
CN¥9,997.65

CN¥1,167.96

Available to ship onApril 25, 2025Details

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100 ML
CN¥1,167.96
250 ML
CN¥2,915.19
4 X 100 ML
CN¥5,649.38
800 ML
CN¥8,418.61
1 L
CN¥9,997.65

About This Item

CN¥1,167.96

Available to ship onApril 25, 2025Details

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)
mobile phase: methanol
flow rate: 1.5 mL/min
pressure: 3249 psi (224 bar)
column temp.: 30 °C
detector: UV, 280 nm
injection: 5 μL
sample: 0.5 mg/mL tocopherol acetate, alpha-tocopherol, delta-tocopherol and K1, 0.05 mg/mL D2, D3 and retinol acetate, 1.5 mg/mL retinol palmitate, all in ethanol.

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

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Tetrabutylammonium hydroxide solution 1.0 M in methanol

230189

Tetrabutylammonium hydroxide solution

Tetrabutylammonium hydroxide solution technical, ~40% in H2O (~1.5 M)

86880

Tetrabutylammonium hydroxide solution

Tetrabutylammonium hydroxide solution 40 wt. % in H2O

178780

Tetrabutylammonium hydroxide solution

Tetrabutylammonium hydroxide solution 53.5-56.5% in H2O

86863

Tetrabutylammonium hydroxide solution

concentration

1.0 M in methanol

concentration

~40% in H2O (~1.5 M)

concentration

40 wt. % in H2O

concentration

53.5-56.5% in H2O

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

liquid

form

liquid

form

liquid

form

liquid

refractive index

n20/D 1.3775

refractive index

-

refractive index

n20/D 1.405

refractive index

-

density

0.83 g/mL at 25 °C

density

0.995 g/cm3

density

0.99 g/mL at 25 °C

density

0.995 g/cm3

greener alternative product characteristics

Catalysis
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

-

greener alternative product characteristics

Catalysis
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

-

Analysis Note

Analysis was conducted using standard solution, detection via UV.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Retinyl acetate, analytical standard
    δ-Tocopherol, analytical standard
    Ergocalciferol (D2), analytical standard
    Cholecalciferol (D3), analytical standard
    (±)-α-Tocopherol, analytical standard
    DL-alpha-Tocopherol acetate, analytical standard
    Phylloquinone (K1), analytical standard
    Retinol palmitate, analytical standard
    See All (8)
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