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Merck
CN

47763

Cholecalciferol (D3)

analytical standard

Synonym(s):

Cholecalciferol, (+)-Vitamin D3, 7-Dehydrocholesterol activated, Activated 7-dehydrocholesterol, Calciol

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About This Item

Empirical Formula (Hill Notation):
C27H44O
CAS Number:
67-97-0
Molecular Weight:
384.64
EC Number:
200-673-2
UNSPSC Code:
12164500
PubChem Substance ID:
24871395
Beilstein/REAXYS Number:
2339331
MDL number:
MFCD00078131
NACRES:
NA.24

Key Documents

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Product Name

Cholecalciferol (D3), analytical standard

InChI key

QYSXJUFSXHHAJI-YRZJJWOYSA-N

InChI

1S/C27H44O/c1-19(2)8-6-9-21(4)25-15-16-26-22(10-7-17-27(25,26)5)12-13-23-18-24(28)14-11-20(23)3/h12-13,19,21,24-26,28H,3,6-11,14-18H2,1-2,4-5H3/b22-12+,23-13-/t21-,24+,25-,26+,27-/m1/s1

SMILES string

CC(C)CCC[C@@H](C)[C@@]1([H])CC[C@@]([C@]1(C)CCC/2)([H])C2=C\C=C(C[C@@H](O)CC3)/C3=C

grade

analytical standard

API family

cholecalciferol

form

solid

CofA

current certificate can be downloaded

feature

standard type fat soluble vitamin

packaging

ampule of 100 mg

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

color

white

mp

83-86 °C (lit.)

application(s)

food and beverages

format

neat

storage temp.

2-8°C

Quality Level

100

Gene Information

human ... VDR(7421)

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Application

Cholecalciferol (D3) may be used as an analytical reference for the quantification of the analyte in different food matrices using different chromatography techniques.
Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Biochem/physiol Actions

Vitamin D acts through a receptor that is a member of the ligand-dependent transcription factor superfamily. Modulates the proliferation and differentiation of both normal and cancer cells. Has antiproliferative and antimetastatic effects on breast, colon, and prostate cancer cells. Activated vitamin D receptors in intestine and bone maintain calcium absorbance and homeostasis.
Vitamin D acts through a receptor that modulates the proliferation and differentiation of both normal and cancer cells.

General description

Cholecalciferol (D3) is a fat soluble vitamin, and is one of the forms of vitamin D, synthesized in human skin on exposure to ultraviolet (UV) radiation from sunlight, or it can also be obtained from food. It is found to be essential in humans for maintaining normal calcium metabolism.

Other Notes

Find a digital Reference Material for this product available on our online platform ChemisTwin® for NMR. You can use this digital equivalent on ChemisTwin® for your sample identity confirmation and compound quantification (with digital external standard). An NMR spectrum of this substance can be viewed and an online comparison against your sample can be performed with a few mouseclicks. Learn more here and start your free trial.

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - STOT RE 1 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
LRAD3064
LRAD9016
LRAC5975
LRAC4188
LC26463V

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Rapid determination of vitamin D3 in milk-based infant formulas by liquid chromatography-tandem mass spectrometry
Kwak M-B, et al.
Food Chemistry, 165(2), 569-574 (2014)
Simultaneous determination of cholecalciferol (vitamin D3) and ergocalciferol (vitamin D2) in foods by selected reaction monitoring
Dimartino G
Journal of AOAC (Association of Official Analytical Chemists) International, 92(2), 511-517 (2009)
Sara S Oberhelman et al.
Mayo Clinic proceedings, 88(12), 1378-1387 (2013-12-03)
To determine whether a single monthly supplement is as effective as a daily maternal supplement in increasing breast milk vitamin D to achieve vitamin D sufficiency in their infants. Forty mothers with exclusively breast-fed infants were randomized to receive oral
Sina Gallo et al.
JAMA, 309(17), 1785-1792 (2013-05-02)
Vitamin D supplementation in infancy is required to support healthy bone mineral accretion. A supplement of 400 IU of vitamin D per day is thought to support plasma 25-hydroxyvitamin D (25[OH]D) concentrations between 40 and 50 nmol/L; some advocate 75
Bich Tran et al.
The American journal of clinical nutrition, 99(1), 156-161 (2013-10-11)
Observational data suggested that supplementation with vitamin D could reduce risk of infection, but trial data are inconsistent. We aimed to examine the effect of oral vitamin D supplementation on antibiotic use. We conducted a post hoc analysis of data
View All Related Papers

Protocols

AOAC Method 2011.11: UHPLC/MS/MS Analysis of Vitamin D2 and Vitamin D3 on Titan™ C18

Separation of Ergocalciferol (D2), analytical standard; Cholecalciferol (D3), analytical standard

HPLC Analysis of Fat Soluble Vitamins by Normal Phase Chromatography on Ascentis® Si

Separation of (±)-α-Tocopherol, analytical standard; Menadione (K3), analytical standard; (+)-γ-Tocopherol, analytical standard; Cholecalciferol (D3), analytical standard

HPLC Analysis of Vitamin D2 and D3

Ascentis Express C18 is suitable for routine analysis of fat-soluble vitamins D2 and D3, providing optimized chromatograms.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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