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581324-U

Supelco

Ascentis® C18 (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

Synonym(s):

Ascentis RP18 HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

Product Name

Ascentis® C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

Quality Level

100

Agency

suitable for USP L1 (Similar to Phenomenex Luna C18)

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

extent of labeling

25% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 4.6 mm

surface area

450 m2/g

surface coverage

3.7 μmol/m2

impurities

<5 ppm metals

matrix

fully porous particle
silica gel high purity, spherical

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

100 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

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General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.

Application


  • Preparation of polar embedded C18 stationary phase for efficient separation of peptides and proteins in high performance liquid chromatography.: This study details the development of a polar embedded C18 stationary phase, demonstrating its effectiveness in separating peptides and proteins. The Ascentis® C18 HPLC Column was utilized for its high separation efficiency, crucial for proteomics and biopharmaceutical applications (Ali et al., 2022).

  • A dilute-and-shoot liquid chromatography-tandem mass spectrometry method for urinary 18-hydroxycortisol quantification and its application in establishing reference intervals.: Researchers used the Ascentis® C18 HPLC Column to develop a sensitive method for quantifying urinary 18-hydroxycortisol. This method aids in clinical diagnostics and endocrinological studies by providing accurate reference intervals (Zhao et al., 2022).

  • A Comparative Study on Analysis of Ginsenosides in American Ginseng Root Residue by HPLC-DAD-ESI-MS and UPLC-HRMS-MS/MS.: This comparative analysis utilized the Ascentis® C18 HPLC Column to separate and identify ginsenosides in American ginseng. The study highlights the column′s application in natural product research and quality control in the pharmaceutical industry (Hsu et al., 2022).

  • Validation of a Rapid and Easy-to-Apply Method to Simultaneously Quantify Co-Loaded Dexamethasone and Melatonin PLGA Microspheres by HPLC-UV: Encapsulation Efficiency and In Vitro Release.: This research validates an HPLC method using the Ascentis® C18 Column to simultaneously quantify dexamethasone and melatonin in PLGA microspheres, offering a robust tool for drug delivery studies (Brugnera et al., 2022).

  • Simultaneous determination of ivermectin, clorsulon and their related substances in an injectable finished product by a stability-indicating RP-HPLC method.: The Ascentis® C18 HPLC Column was employed to develop a stability-indicating method for analyzing ivermectin and clorsulon in pharmaceutical formulations, emphasizing its role in quality control and regulatory compliance (Padivitage et al., 2022).

Features and Benefits

  • Excellent retention
  • Symmetric peak shape
  • High reproducibility
  • Complete LC-MS compatibility

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
292982-06
292982-05
292982-04
292982-03
292982-02

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the storage solvent for Product 581324-U, Ascentis® C18 HPLC Column - 5 ?m particle size, L × I.D. 15 cm × 4.6 mm?

    The storage solvent is a ratio of 40:60 water:acetonitrile for this column.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. What flow rate should I use with Ascentis® Express HPLC Columns?

    Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID;  0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.

  7. Can Ascentis® Express HPLC Columns be used for LC-MS?

    Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Federica Vacondio et al.
Journal of pharmaceutical and biomedical analysis, 46(1), 200-205 (2007-10-26)
A rapid, simple and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the determination of the imidazole H(3) antagonist ROS203 in rat plasma, using the superior homologue ROS287 as internal standard. Analyses were performed on an Agilent
Dipanjan Goswami et al.
Biomedical chromatography : BMC, 23(11), 1227-1241 (2009-07-14)
A LC-MS/MS method for plasma topiramate analysis is delineated involving least number of healthy volunteers. Topiramate and amlodipine internal standard (IS) were extracted by simple centrifuge-coupled solid-phase extraction and reverse-phase chromatographic separation was performed on an Ascentis C(18) column. Turbo-spray
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 55(5), 934-948 (2011-04-19)
In this study, the composition of polyphenols (phenolic acids and flavonoids) in propolis extracts was investigated by HPLC-DAD and HPLC-ESI-MS/MS by comparing the performance of ion trap and triple quadrupole mass analyzers. The analyses were carried out on an Ascentis
Federica Pellati et al.
Journal of chromatography. A, 1242, 43-58 (2012-05-09)
In this study, a detailed phytochemical characterization of Echinacea pallida (Nutt.) Nutt. root extracts and dietary supplements was carried out for the first time by developing advanced chromatographic techniques, based on HPLC with diode array (DAD) and electrospray ionization-mass spectrometry
Meiyun Shi et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 951-952, 129-134 (2014-02-22)
Aralia mandshrica is a well-known traditional Chinese medicine from Northeast China commonly used to treat digestive, circulatory and immune system disorders. Calenduloside E is one of its bioactive components currently under evaluation as a pure drug. In this study, a
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Protocols

Analysis of Polar Compounds with Ion Pair Reagents

Reversed-phase HPLC sometimes requires ion pairing for adequate retention of polar compounds due to hydrophobic interactions.

HPLC Analysis of Warfarin Anticoagulant on Ascentis C18

HPLC Analysis of Warfarin™ Anticoagulant on Ascentis® C18

Chromatograms

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