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581322-U

Supelco

Ascentis® C18 (3 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

Synonym(s):

Ascentis RP18 HPLC Column

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Select a Size

5 G
¥764.47
10 G
¥1,483.07
25 G
¥3,038.93
100 G
¥9,154.39
1 KG
¥48,652.34

¥764.47

Please contact Customer Service for Availability
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Select a Size

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5 G
¥764.47
10 G
¥1,483.07
25 G
¥3,038.93
100 G
¥9,154.39
1 KG
¥48,652.34

About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

¥764.47

Please contact Customer Service for Availability

Product Name

Ascentis® C18 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

Quality Level

100

Agency

suitable for USP L1 (Similar to Phenomenex Luna C18)

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

extent of labeling

25% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

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ZTC18SZTSCXSZTC18M
ZipTip®移液器吸头 C4 resin, bed volume 0.6 μL, tip volume 10 μL

ZTC04S

ZipTip®移液器吸头

ZipTip® 移液器吸头 C18 resin, bed volume 0.6 μL, tip volume 10 μL

ZTC18S

ZipTip® 移液器吸头

ZipTip® 移液器吸头 strong cation exchange (SCX) resin, bed volume 0.6 μL, tip volume 10 μL

ZTSCXS

ZipTip® 移液器吸头

ZipTip® 移液器吸头 C18 resin, micro, bed volume 0.2 μL, tip volume 10 μL

ZTC18M

ZipTip® 移液器吸头

manufacturer/tradename

ZipTip®

manufacturer/tradename

ZipTip®

manufacturer/tradename

ZipTip®

manufacturer/tradename

ZipTip®

material

polypropylene , silica resin

material

polypropylene

material

polypropylene , resin (Strong cation exchange)

material

polypropylene

parameter

4-70  °C temperature

parameter

4-70  °C temperature

parameter

4-70  °C temperature

parameter

4-70  °C temperature

technique(s)

analytical sample preparation: suitable

technique(s)

analytical sample preparation: suitable, fractionation: suitable, protein purification: suitable (concentration)

technique(s)

analytical sample preparation: suitable, fractionation: suitable, protein purification: suitable (concentration)

technique(s)

analytical sample preparation: suitable, fractionation: suitable, protein purification: suitable (concentration)

bed volume

0.6 μL

bed volume

0.6 μL

bed volume

0.6 μL

bed volume

0.2 μL

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.

Application


  • A dilute-and-shoot liquid chromatography-tandem mass spectrometry method for urinary 18-hydroxycortisol quantification and its application in establishing reference intervals: This paper details the application of Ascentis® C18 columns in a clinical setting to measure cortisol levels in urine. The method provides a fast, accurate, and reliable way to assess patient hormonal profiles, critical for diagnosing endocrine disorders (Zhao et al., 2022).

  • A Comparative Study on Analysis of Ginsenosides in American Ginseng Root Residue by HPLC-DAD-ESI-MS and UPLC-HRMS-MS/MS: Utilizing Ascentis® C18, this study compares different analytical methods for quantifying ginsenosides. It showcases the column′s adaptability and precision in natural product research, essential for ensuring the quality and efficacy of herbal medicines (Hsu et al., 2022).

  • Validation of a Rapid and Easy-to-Apply Method to Simultaneously Quantify Co-Loaded Dexamethasone and Melatonin PLGA Microspheres by HPLC-UV: Highlighting the Ascentis® C18′s effectiveness in pharmaceutical formulations, this research demonstrates its capacity to analyze complex drug delivery systems, contributing to the development of more effective therapeutic solutions (Brugnera et al., 2022).

  • Simultaneous determination of ivermectin, clorsulon and their related substances in an injectable finished product by a stability-indicating RP-HPLC method: This publication illustrates the application of the Ascentis® C18 in veterinary medicine, providing crucial insights into drug stability and composition which are vital for regulatory compliance and animal safety (Padivitage et al., 2022).

Features and Benefits

  • Excellent retention
  • Symmetric peak shape
  • High reproducibility
  • Complete LC-MS compatibility

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    292159-06
    292159-05
    292159-04
    292159-03
    292159-02

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    Federica Pellati et al.
    Journal of chromatography. A, 1242, 43-58 (2012-05-09)
    In this study, a detailed phytochemical characterization of Echinacea pallida (Nutt.) Nutt. root extracts and dietary supplements was carried out for the first time by developing advanced chromatographic techniques, based on HPLC with diode array (DAD) and electrospray ionization-mass spectrometry
    Dipanjan Goswami et al.
    Biomedical chromatography : BMC, 23(11), 1227-1241 (2009-07-14)
    A LC-MS/MS method for plasma topiramate analysis is delineated involving least number of healthy volunteers. Topiramate and amlodipine internal standard (IS) were extracted by simple centrifuge-coupled solid-phase extraction and reverse-phase chromatographic separation was performed on an Ascentis C(18) column. Turbo-spray
    Federica Pellati et al.
    Journal of pharmaceutical and biomedical analysis, 55(5), 934-948 (2011-04-19)
    In this study, the composition of polyphenols (phenolic acids and flavonoids) in propolis extracts was investigated by HPLC-DAD and HPLC-ESI-MS/MS by comparing the performance of ion trap and triple quadrupole mass analyzers. The analyses were carried out on an Ascentis
    Meiyun Shi et al.
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 951-952, 129-134 (2014-02-22)
    Aralia mandshrica is a well-known traditional Chinese medicine from Northeast China commonly used to treat digestive, circulatory and immune system disorders. Calenduloside E is one of its bioactive components currently under evaluation as a pure drug. In this study, a
    Federica Vacondio et al.
    Journal of pharmaceutical and biomedical analysis, 46(1), 200-205 (2007-10-26)
    A rapid, simple and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the determination of the imidazole H(3) antagonist ROS203 in rat plasma, using the superior homologue ROS287 as internal standard. Analyses were performed on an Agilent
    View All Related Papers

    Articles

    Bioethanol

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    Protocols

    US EPA Method 632: HPLC Analysis of Pesticides on Ascentis® C18

    -methylcarbamate 10 μg/mL; Diuron; Propham; Siduron; Methiocarb, analytical standard; Linuron 10 μg/mL; Swep 10 μg/mL; Chlorpropham 10 μg/mL; Barban; Neburon

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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