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581325-U

Supelco

Ascentis® C18 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

Synonym(s):

Ascentis RP18 HPLC Column

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1 EA
CN¥6,148.65

CN¥6,148.65

Available to ship onApril 27, 2025Details

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1 EA
CN¥6,148.65

About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

CN¥6,148.65

Available to ship onApril 27, 2025Details

Product Name

Ascentis® C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

material

stainless steel column

Quality Level

100

Agency

suitable for USP L1 (Similar to Phenomenex Luna C18)

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

extent of labeling

25% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

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This Item
581322U581321U581301U
Ascentis® C18 HPLC Column 5 μm particle size, L × I.D. 25 cm × 4.6 mm

581325U

Ascentis® C18 HPLC Column

Ascentis® C18 HPLC Column 3 μm particle size, L × I.D. 15 cm × 4.6 mm

581322U

Ascentis® C18 HPLC Column

Ascentis® C18 HPLC Column 3 μm particle size, L × I.D. 10 cm × 4.6 mm

581321U

Ascentis® C18 HPLC Column

Ascentis® C18 HPLC Column 3 μm particle size, L × I.D. 10 cm × 2.1 mm

581301U

Ascentis® C18 HPLC Column

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix

fully porous particle, silica gel high purity, spherical

matrix

fully porous particle, silica gel high purity, spherical

matrix

fully porous particle, silica gel high purity, spherical

matrix

fully porous particle, silica gel high purity, spherical

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

product line

Ascentis®

product line

Ascentis®

product line

Ascentis®

product line

Ascentis®

pore size

100 Å

pore size

100 Å

pore size

100 Å

pore size

100 Å

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.

Application


  • Identification of recombinant human insulin and biosynthetic insulin analogues by multiplexed targeted unlabeled mass spectrometry of proteotypic tryptic peptides.: This study utilized the Ascentis® C18 HPLC column for the separation of proteotypic tryptic peptides in the identification of insulin analogues, highlighting its application in targeted mass spectrometry for proteomic analysis (Qasem RJ et al., 2019).

  • Development and validation of a novel stability-indicating HPLC method for the simultaneous assay of betamethasone-17-valerate, fusidic acid, potassium sorbate, methylparaben and propylparaben in a topical cream preparation.: This research developed and validated an HPLC method using the Ascentis® C18 HPLC column for the simultaneous assay of multiple compounds, demonstrating its robustness in stability testing of pharmaceutical formulations (Byrne J et al., 2014).

  • HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts.: The study employed the Ascentis® C18 HPLC column for metabolite profiling of propolis extracts using HPLC-DAD and HPLC-ESI-MS/MS, showcasing its effectiveness in complex natural product analysis (Pellati F et al., 2011).

  • Practical comparison of 2.7 microm fused-core silica particles and porous sub-2 microm particles for fast separations in pharmaceutical process development.: This article compared different HPLC column technologies, including the Ascentis® C18, highlighting its performance in fast separations critical for pharmaceutical process development (Abrahim A et al., 2010).

Features and Benefits

  • Excellent retention
  • Symmetric peak shape
  • High reproducibility
  • Complete LC-MS compatibility

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    295475-06
    295475-05
    295475-04
    295475-03
    295475-02

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    Michael Kühl et al.
    eLife, 9 (2020-01-22)
    Far-red absorbing chlorophylls are constitutively present as chlorophyll (Chl) d in the cyanobacterium Acaryochloris marina, or dynamically expressed by synthesis of Chl f, red-shifted phycobiliproteins and minor amounts of Chl d via far-red light photoacclimation in a range of cyanobacteria
    Federica Vacondio et al.
    Journal of pharmaceutical and biomedical analysis, 46(1), 200-205 (2007-10-26)
    A rapid, simple and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the determination of the imidazole H(3) antagonist ROS203 in rat plasma, using the superior homologue ROS287 as internal standard. Analyses were performed on an Agilent
    Federica Pellati et al.
    Journal of pharmaceutical and biomedical analysis, 55(5), 934-948 (2011-04-19)
    In this study, the composition of polyphenols (phenolic acids and flavonoids) in propolis extracts was investigated by HPLC-DAD and HPLC-ESI-MS/MS by comparing the performance of ion trap and triple quadrupole mass analyzers. The analyses were carried out on an Ascentis
    Dipanjan Goswami et al.
    Biomedical chromatography : BMC, 23(11), 1227-1241 (2009-07-14)
    A LC-MS/MS method for plasma topiramate analysis is delineated involving least number of healthy volunteers. Topiramate and amlodipine internal standard (IS) were extracted by simple centrifuge-coupled solid-phase extraction and reverse-phase chromatographic separation was performed on an Ascentis C(18) column. Turbo-spray
    Federica Pellati et al.
    Journal of chromatography. A, 1242, 43-58 (2012-05-09)
    In this study, a detailed phytochemical characterization of Echinacea pallida (Nutt.) Nutt. root extracts and dietary supplements was carried out for the first time by developing advanced chromatographic techniques, based on HPLC with diode array (DAD) and electrospray ionization-mass spectrometry
    View All Related Papers

    Protocols

    US EPA Method 632: HPLC Analysis of Pesticides on Ascentis® C18

    -methylcarbamate 10 μg/mL; Diuron; Propham; Siduron; Methiocarb, analytical standard; Linuron 10 μg/mL; Swep 10 μg/mL; Chlorpropham 10 μg/mL; Barban; Neburon

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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