HPLC Analysis of Benzoic Acid Derivatives on Ascentis^® C18

column

Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)

mobile phase

[A] 10mM TFA in water; [B] 10 mM TFA in acetonitrile; (70:30, A:B)

flow rate

1.0 mL/min

column temp.

30 °C

detector

UV, 220 nm

injection

10 μL