Skip to Content
Merck
CN
All Photos(1)

Documents

565324-U

Supelco

Ascentis® RP-Amide (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

Synonym(s):

RP-Amide Reversed-Phase Chromatography Column

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® RP-Amide HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

Quality Level

100

Agency

suitable for USP L60

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

extent of labeling

19.5% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 4.6 mm

surface area

450 m2/g

surface coverage

2.7 μmol/m2

impurities

<5 ppm metals

matrix

fully porous particle
silica gel high purity, spherical

matrix active group

amide, alkyl phase

particle size

5 μm

pore size

100 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.

Application


  • Preparation of mixed-mode stationary phase for separation of peptides and proteins in high-performance liquid chromatography: Highlights the application of Ascentis® RP-Amide HPLC column in the development of mixed-mode stationary phases, further demonstrating its versatility in handling diverse biochemical compounds and its pivotal role in advancing pharmaceutical and proteomic research (Alharthi et al., 2022).

  • On-line hyphenation of solid-phase extraction to chromatographic separation of sulfonamides with fused-core columns in sequential injection chromatography: Showcases the efficacy of Ascentis® RP-Amide HPLC columns in environmental analysis, specifically for the detection of contaminants in complex matrices. This study underscores the column′s robustness and high-resolution capabilities, making it invaluable in environmental laboratories (Batista et al., 2015).

  • Determination of zearalenone and its metabolites in endometrial cancer by coupled separation techniques: Demonstrates the critical role of Ascentis® RP-Amide HPLC columns in clinical research, particularly in the sensitive and accurate measurement of biomarkers in cancer diagnostics. The column′s superior retention for polar compounds proves essential for precise biomedical analyses (Gadzała-Kopciuch et al., 2011).

Features and Benefits

  • Excellent retention and peak shape for polar compounds
  • 100% aqueous compatibility
  • Ultra low bleed, LC-MS compatible
  • Unique selectivity

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

Product No.
Description
Pricing

guard cartridge

Product No.
Description
Pricing

related product

Product No.
Description
Pricing

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
292317-06
292317-05
292317-04
292317-03
292317-02

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 151, 291-300 (2018-02-08)
The presented work describes the development and validation of a rapid UHPLC-UV method using a fused core particle column with an RP-Amide stationary phase for the separation and quantitative analysis of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. Three
Rihana Parveen Shaik et al.
Journal of pharmaceutical analysis, 3(6), 489-499 (2013-12-01)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for simultaneous quantification of tolterodine and its metabolite 5-hydroxy methyl tolterodine in rat plasma. Tolterodine-d6 and 5-hydroxy methyl tolterodine-d14 were used as internal standards (IS). Chromatographic separation was performed on Ascentis Express

Articles

Reference Standards for Analyzing Polyphenol Catechins

Reference Standards for Analyzing Polyphenol Catechins

Protocols

Mass Spectral Column Bleed in Nitrogen-Containing Polar-Embedded HPLC Stationary Phases

Mass Spectral Column Bleed in Nitrogen-Containing Polar-Embedded HPLC Stationary Phases

HPLC Analysis of Flavonoids on Ascentis® RP-Amide

Protocol for HPLC Analysis of Flavonoids on Ascentis® RP-Amide

Chromatograms

application for HPLCapplication for HPLC

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service