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G005676

HPLC Analysis of Benzoic Acid Derivatives on Ascentis® C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)
mobile phase: [A] 10mM TFA in water; [B] 10 mM TFA in acetonitrile; (70:30, A:B)
flow rate: 1.0 mL/min
column temp.: 30 °C
detector: UV, 220 nm
injection: 10 μL

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    4-Hydroxybenzoic acid
    Acetylsalicylic acid
    Benzoic acid, meets USP testing specifications
    Salicylic acid, puriss. p.a., ≥99.0% (T)
    Ethyl 4-hydroxybenzoate, ReagentPlus®, 99%

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