S8633
Sphingomyelinase from Staphylococcus aureus
buffered aqueous glycerol solution, 100-300 units/mg protein (Lowry)
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Synonym(s):
Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase
CAS Number:
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
form
buffered aqueous glycerol solution
Quality Level
specific activity
100-300 units/mg protein (Lowry)
storage temp.
2-8°C
Application
Sphingomyelinase from Staphylococcus aureus has been used to:
- induce neurotoxicity in rat cortical cultures to study the protective effects of minocycline
- determine the concentration of sphingomyelin from serum samples
- enhance sphingomyelinase activity to study PARK9-mediated exosome biogenesis
Biochem/physiol Actions
Bacterial sphingomyelinase is active at neutral pH. When used in cell culture in vitro, it hydrolyzes the sphingomyelin on the outer leaflet of the plasma membrane and produces ceramide that is lipid-soluble. Sphingomyelinase is the key enzyme in the sphingomyelinase/ceramide pathway, which is implicated in the pathogenesis of several neurodegenerative disorders.
Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.
Unit Definition
One unit will hydrolyze 1.0 μmol of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.
Physical form
Solution in 50% glycerol containing 0.25 M phosphate buffer, pH 7.5
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Measurement of sphingomyelin and ceramide cellular levels after sphingomyelinase-mediated sphingomyelin hydrolysis.
P Santana et al.
Methods in molecular biology (Clifton, N.J.), 105, 217-221 (1999-07-31)
Elin Rebecka Carlsson et al.
Frontiers in endocrinology, 9, 172-172 (2018-06-21)
Metabolic surgery is superior to lifestyle intervention in reducing weight and lowering glycemia and recently suggested as treatment for type 2 diabetes mellitus. Especially Roux-en-Y gastric bypass (RYGB) has been focus for much research, but still the mechanisms of action
Taiji Tsunemi et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(46), 15281-15287 (2014-11-14)
Kufor-Rakeb syndrome (KRS) is caused by loss-of-function mutations in ATP13A2 (PARK9) and characterized by juvenile-onset parkinsonism, pyramidal signs, and cognitive decline. Previous studies suggested that PARK9 deficiency causes lysosomal dysfunction and α-synuclein (α-syn) accumulation, whereas PARK9 overexpression suppresses toxicity of
Hyunae Kim et al.
Nutrition research (New York, N.Y.), 32(12), 956-964 (2012-12-19)
We have previously reported that dietary sericin improves epidermal dryness with the increased total Ceramide (Cer) in NC/Nga mice, an animal model of atopic dermatitis (AD). In this study, we hypothesized that the increased level of total Cer induced by
Feixiang Wang et al.
The Journal of clinical investigation, 131(19) (2021-08-18)
Proper metabolic activities facilitate T cell expansion and antitumor function; however, the mechanisms underlying disruption of the T cell metabolic program and function in the tumor microenvironment (TME) remain elusive. Here, we show a zinc finger protein 91-governed (ZFP91-governed) mechanism
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