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Key Documents

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Safety Information

S8633

Sigma-Aldrich

Sphingomyelinase from Staphylococcus aureus

buffered aqueous glycerol solution, 100-300 units/mg protein (Lowry)

Synonym(s):

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

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About This Item

CAS Number:
9031-54-3
Enzyme Commission number:
3.1.4.12 (BRENDA, IUBMB)
MDL number:
MFCD00132362
UNSPSC Code:
12352204
NACRES:
NA.32
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form

buffered aqueous glycerol solution

Quality Level

200

specific activity

100-300 units/mg protein (Lowry)

storage temp.

2-8°C

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application(s)

cleaning products
clinical
cosmetics
environmental
food and beverages
forensics and toxicology
personal care
pharmaceutical (small molecule)

application(s)

pharmaceutical (small molecule)

application(s)

-

application(s)

-

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

technique(s)

-

format

neat

format

neat

format

-

format

-

Quality Level

100

Quality Level

300

Quality Level

100

Quality Level

200

grade

analytical standard

grade

certified reference material, pharmaceutical secondary standard

grade

-

grade

-

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

-

shelf life

-

Application

Sphingomyelinase from Staphylococcus aureus has been used to:
  • induce neurotoxicity in rat cortical cultures to study the protective effects of minocycline[1]
  • determine the concentration of sphingomyelin from serum samples[2]
  • enhance sphingomyelinase activity to study PARK9-mediated exosome biogenesis[3]

Biochem/physiol Actions

Bacterial sphingomyelinase is active at neutral pH. When used in cell culture in vitro, it hydrolyzes the sphingomyelin on the outer leaflet of the plasma membrane and produces ceramide that is lipid-soluble. Sphingomyelinase is the key enzyme in the sphingomyelinase/ceramide pathway, which is implicated in the pathogenesis of several neurodegenerative disorders.[1]
Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Unit Definition

One unit will hydrolyze 1.0 μmol of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

Physical form

Solution in 50% glycerol containing 0.25 M phosphate buffer, pH 7.5

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000408047
    0000381918
    0000332136
    0000332135
    0000302600

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    Measurement of sphingomyelin and ceramide cellular levels after sphingomyelinase-mediated sphingomyelin hydrolysis.
    P Santana et al.
    Methods in molecular biology (Clifton, N.J.), 105, 217-221 (1999-07-31)
    Elin Rebecka Carlsson et al.
    Frontiers in endocrinology, 9, 172-172 (2018-06-21)
    Metabolic surgery is superior to lifestyle intervention in reducing weight and lowering glycemia and recently suggested as treatment for type 2 diabetes mellitus. Especially Roux-en-Y gastric bypass (RYGB) has been focus for much research, but still the mechanisms of action
    Taiji Tsunemi et al.
    The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(46), 15281-15287 (2014-11-14)
    Kufor-Rakeb syndrome (KRS) is caused by loss-of-function mutations in ATP13A2 (PARK9) and characterized by juvenile-onset parkinsonism, pyramidal signs, and cognitive decline. Previous studies suggested that PARK9 deficiency causes lysosomal dysfunction and α-synuclein (α-syn) accumulation, whereas PARK9 overexpression suppresses toxicity of
    Feixiang Wang et al.
    The Journal of clinical investigation, 131(19) (2021-08-18)
    Proper metabolic activities facilitate T cell expansion and antitumor function; however, the mechanisms underlying disruption of the T cell metabolic program and function in the tumor microenvironment (TME) remain elusive. Here, we show a zinc finger protein 91-governed (ZFP91-governed) mechanism
    Yuki Katayama et al.
    Journal of bacteriology, 195(6), 1194-1203 (2013-01-08)
    Colonization by Staphylococcus aureus is a characteristic feature of several inflammatory skin diseases and is often followed by epidermal damage and invasive infection. In this study, we investigated the mechanism of skin colonization by a virulent community-acquired methicillin-resistant S. aureus
    View All Related Papers

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