P4912
Phospholipase D from Streptomyces sp.
Type VII, lyophilized powder, ≥150 units/mg solid
Synonym(s):
Lecithinase D, Phosphatidylcholine phosphatidohydrolase
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
biological source
Streptomyces sp.
Quality Level
type
Type VII
form
lyophilized powder
specific activity
≥150 units/mg solid
storage temp.
−20°C
Application
Phospholipase D (PLD) has been used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to yield the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD has been used in purification and kinetic studies.
The enzyme from Sigma has been used in the translocation of sphingosine kinase 1 (SK1) to membrane fractions under in vitro conditions. It has also been used to produce phosphatidic acid (PA) from phosphatidylcholine (PC) in HL60 permeabilized cells.
Biochem/physiol Actions
Phospholipase D is glycerophospholipid-specific. It is markedly less active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Unit Definition
One unit will liberate 1.0 μmole of choline from 1,2-Dioleoyl-sn-glycero-3-phosphocholine per hour at pH 8.0 at 37 °C.
Analysis Note
Protein by biuret
inhibitor
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Fava beans can substitute soybean meal and rapeseed meal as protein source in diets for lactating dairy cows.
Hansen, et al.
Journal of Dairy Science, 104, 5508-5521 (2021)
Christine Delon et al.
The Journal of biological chemistry, 279(43), 44763-44774 (2004-08-18)
Sphingosine kinase 1 (SK1) phosphorylates sphingosine to generate sphingosine 1-phosphate (S1P). Because both substrate and product of the enzyme are potentially important signaling molecules, the regulation of SK1 is of considerable interest. We report that SK1, which is ordinarily a
Mohamed H Yaghmour et al.
Journal of lipid research, 62, 100022-100022 (2021-01-17)
Phospholipids with a choline head group are an abundant component of cellular membranes and are involved in many important biological functions. For studies on the cell biology and metabolism of these lipids, traceable analogues where propargylcholine replaces the choline head
Kathryn Garner et al.
The Journal of biological chemistry, 287(38), 32263-32276 (2012-07-24)
Phosphatidylinositol transfer proteins (PITPs) are versatile proteins required for signal transduction and membrane traffic. The best characterized mammalian PITPs are the Class I PITPs, PITPα (PITPNA) and PITPβ (PITPNB), which are single domain proteins with a hydrophobic cavity that binds
Viviana Trezza et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(43), 14899-14908 (2012-10-27)
The brain endocannabinoid system plays a crucial role in emotional processes. We have previously identified an important role for endocannabinoids in social play behavior, a highly rewarding form of social interaction in adolescent rats. Here, we tested the hypothesis that
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service