S9396
Sphingomyelinase from Bacillus cereus
buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)
Synonym(s):
Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase
Sign In to View Organizational & Contract Pricing
Select a Size
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
form
buffered aqueous glycerol solution
Quality Level
specific activity
≥100 units/mg protein (Lowry)
mol wt
34.2 kDa
foreign activity
Phospholipase C ≤1%
storage temp.
2-8°C
Gene Information
Bacillus cereus ATCC 10987 ... spH(2751530)
Looking for similar products? Visit Product Comparison Guide
General description
Sphingomyelinase (SMase) is a hydrolase, of 34.2 kDa and its active site contain as a minimum of two magnesium atoms.
Application
Sphingomyelinase from Bacillus cereus has been used:
- in the study to analyse the effect of sphingomyelinase on Rubella virus infectivity in cell lines
- in lipoprotein modification for the generation of cholesterol crystals in vitro
- to aggregate LDL (low-density lipoprotein)
Sphingomyelinase has been used in a study to assess the interaction of actin with the HIV-1 accessory protein Nef. Sphingomyelinase has also been used in a study to investigate X-ray scattering as a quality-control tool for liposomal drug-delivery systems.
Biochem/physiol Actions
Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.
Sphingomyelin phosphodiesterase (SMPD) participates in the metabolic reactions comprising sphingomyelin. SMPD has the ability to prevent colorectal carcinoma and colitis.
Physical form
Solution in 50% glycerol containing 50 mM Tris-HCl, pH 7.5.
Other Notes
One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
G Pabst et al.
European biophysics journal : EBJ, 41(10), 915-929 (2012-06-05)
Biological membranes can be targets for compounds that either disrupt their barrier function or affect protein function via membrane-mediated processes. Biophysical studies on membrane-mimetic systems composed of membrane lipids have contributed substantially to our knowledge on the pertaining membrane physics
The Bacillus cereus group
Molecular Medical Microbiology, 1041-1078 (2015)
Analysis of the Effect of Sphingomyelinase on Rubella Virus Infectivity in Two Cell Lines
Otsuki N, et al.
Journal of virology (2017)
Extracellular lipids accumulate in human carotid arteries as distinct three-dimensional structures and have proinflammatory properties
Lehti S, et al.
The American Journal of Pathology, 188(2), 525-538 (2018)
Antioxidants inhibit low density lipoprotein oxidation less at lysosomal pH: A possible explanation as to why the clinical trials of antioxidants might have failed
Ahmad F and Leake D S
Chemistry and Physics of Lipids, 213, 13-24 (2018)
Related Content
Instructions
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service