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Safety Information

OGS109

Sigma-Aldrich

PSF-CMV-PGEM - CMV PGEM MCS PLASMID

plasmid vector for molecular cloning

Synonym(s):

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.85

form

buffered aqueous solution

mol wt

size 4354 bp

bacteria selection

ampicillin

Origin of replication

pUC (500 copies)

Peptide cleavage

no cleavage

Promoter

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

reporter gene

none

shipped in

ambient

storage temp.

−20°C

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PSF-CMV-PGEM - CMV PGEM MCS PLASMID plasmid vector for molecular cloning

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PSF-CMV-PGEM - CMV PGEM MCS PLASMID

PSF-CMV-PUC18 - CMV PUC18 MCS PLASMID plasmid vector for molecular cloning

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PSF-CMV-PUC18 - CMV PUC18 MCS PLASMID

PSF-CMV-AMP - CMV PROMOTER PLASMID plasmid vector for molecular cloning

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PSF-CMV-AMP - CMV PROMOTER PLASMID

PSF-CMV-SEAP - CMV SEAP PLASMID plasmid vector for molecular cloning

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PSF-CMV-SEAP - CMV SEAP PLASMID

peptide cleavage

no cleavage

peptide cleavage

no cleavage

peptide cleavage

no cleavage

peptide cleavage

no cleavage

promoter

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

promoter

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

promoter

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

promoter

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

bacteria selection

ampicillin

bacteria selection

ampicillin

bacteria selection

ampicillin

bacteria selection

ampicillin

mol wt

size 4354 bp

mol wt

size 4310 bp

mol wt

size 4318 bp

mol wt

size 5849 bp

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

General description

PSF-CMV-PGEM -CMV PGEM MCS plasmid is a versatile cloning vector for the expression of genes in mammalian cells. The molecular cloning vector contains the multiple cloning site (MCS) from the Promega pGEM vector however it has been modified slightly to accommodate some restriction sites in the SnapFast system. These changes are described in the cloning tab. The use of this MCS plasmid instead of the normal SnapFast vector MCS will limit the ability to use some of the inserts that we sell in other plasmid that immediately flank or are inserted within the standard MCS. In CMV PGEM MCS plasmid, these limitations mainly extend to 5 prime tags and signal peptides. Most other inserts should still be compatible with this vector. pGEM is a registered trademark of the Promega Corporation.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.

Application

Multiple cloning site notes: This vector contains a different multiple cloning site (MCS) from the standard SnapFast vector. The MCS is derived from pGEM. The order of the restriction sites in this vector are almost the same as in pGEM however the sequence is not. The pGEM MCS has also been modified as follows:

  • The normal NotI SbfI and PstI sites have been ablated in this MCS. The NotI site has been moved to the 5 prime of the MCS this allows the use of many of our 5 prime coding sequences and tags.
  • An XbaI site has been added to the end of the MCS.
  • The BamHI site that is normally found in all SnapFast vectors (in the downstream fusion MCS) has been replaced with a BclI site. BamHI and BclI produce compatible cohesive ends when cleaved although BclI is methylated in this vector and will require growth in a Dam methylase negative bacterial strain (such as JM110 cells) before it can be used.


The ClaI to NheI sites have other functions such as adding peptide tags or IRES elements. The BsgI and BseRI restriction sites cleave within the stop codon in the XbaI site and allow the retrospective fusion of coding sequences. These sites are normally only used on genes that we sell in the main multiple cloning site. This concept is explained in more detail on our website using the drop down box on the homepage and selecting: Fuse a coding sequence to the 3 prime end of a gene already in the SnapFast plasmid.

Sequence

To view sequence information for this product, please visit the product page

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxgene.com

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Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    Alexander C Cerny et al.
    PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)
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    Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
    The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer
    Jin-Gyoung Jung et al.
    PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)
    The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.
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    Carcinogenesis, 37(1), 18-29 (2015-10-28)
    Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we

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