M9956
M9 Minimal Salts, 5X
Liquid microbial growth medium
Synonym(s):
5x M9 salts
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About This Item
UNSPSC Code:
12352302
NACRES:
NA.85
grade
for molecular biology
Quality Level
sterility
sterile; 0.2 μm filtered
form
liquid
suitability
suitable for nonselective for Escherichia coli and Coliforms
storage temp.
room temp
General description
M9 minimal media is a highly-referenced microbial growth medium used for the cultivation of E. coli. This buffered minimal microbial medium contains only salts and nitrogen, so it is traditionally supplemented with glucose, amino acids and vitamins as needed.
Application
M9 Minimal Salts, 5X is suitable for non-selective cultivation of Escherichia coli strains for cloning, DNA plasmid production and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Features and Benefits
M9 minimal media provides:
- Filter-sterilized, ready-to-use format
- Convenient package size
- Standard formulation
Components
15 g/L KH2PO4
64 g/L Na2HPO4 ⋅7H2O
2.5 g/L NaCl
5.0 g/L NH4Cl
64 g/L Na2HPO4 ⋅7H2O
2.5 g/L NaCl
5.0 g/L NH4Cl
related product
Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Effect of culture conditions on microorganism identification by matrix-assisted laser desorption ionization mass spectrometry.
Valentine N, et al.
Applied and Environmental Microbiology, 71(1), 58-64 (2005)
Identification and characterization of a two-component sensor-kinase and response-regulator system (DcuS-DcuR) controlling gene expression in response to C4-dicarboxylates in Escherichia coli.
Golby P, et al.
Journal of Bacteriology, 181(4), 1238-1248 (1999)
Joshua W McCausland et al.
Nature communications, 12(1), 609-609 (2021-01-29)
The FtsZ protein is a central component of the bacterial cell division machinery. It polymerizes at mid-cell and recruits more than 30 proteins to assemble into a macromolecular complex to direct cell wall constriction. FtsZ polymers exhibit treadmilling dynamics, driving
Protocols
Initiating a Starter Culture
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