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Key Documents

M9956

Sigma-Aldrich

M9 Minimal Salts, 5X

Liquid microbial growth medium

Synonym(s):

5x M9 salts

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About This Item

UNSPSC Code:
12352302
NACRES:
NA.85
grade:
Molecular Biology
form:
liquid
Pricing and availability is not currently available.

grade

Molecular Biology

Quality Level

500

sterility

sterile; 0.2 μm filtered

form

liquid

suitability

suitable for nonselective for Escherichia coli and Coliforms

storage temp.

room temp

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biological source

plant (wild yam)

biological source

-

biological source

synthetic (organic)

biological source

-

assay

97.0-102.0% dry basis

assay

-

assay

≥80% (HPLC)

assay

≥98%

Gene Information

human ... ABCB1(5243), CYP3A4(1576), NR3C1(2908), SERPINA6(866)
mouse ... Abcb1a(18671), Abcb1b(18669), Ifng(15978), Nos2(18126)
rat ... Ar(24208), Nr3c1(24413)

Gene Information

human ... NR3C1(2908)

Gene Information

human ... ABCB1(5243), CYP3A4(1576), NR3C1(2908), SERPINA6(866)
mouse ... Abcb1a(18671), Abcb1b(18669), Ifng(15978), Nos2(18126)
rat ... Ar(24208), Nr3c1(24413)

Gene Information

human ... ABCB1(5243), CYP3A4(1576), NR3C1(2908), SERPINA6(866)
mouse ... Abcb1a(18671), Abcb1b(18669), Ifng(15978), Nos2(18126)
rat ... Ar(24208), Nr3c1(24413)

Quality Level

300

Quality Level

300

Quality Level

200

Quality Level

200

form

powder

form

-

form

powder

form

powder

solubility

water: soluble 0.28 g/L at 25 °C

solubility

-

solubility

ethanol: 1 mg/mL, clear

solubility

chloroform/ethanol (1:1): 50 mg/mL, clear, colorless to very faintly yellow

General description

M9 minimal media is a highly-referenced microbial growth medium used for the cultivation of E. coli. This buffered minimal microbial medium contains only salts and nitrogen, so it is traditionally supplemented with glucose, amino acids and vitamins as needed.

Application

M9 Minimal Salts, 5X is suitable for non-selective cultivation of Escherichia coli strains for cloning, DNA plasmid production and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.[1][2]
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

M9 minimal media provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Components

15 g/L KH2PO4
64 g/L Na2HPO4 ⋅7H2O
2.5 g/L NaCl
5.0 g/L NH4Cl

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000336165
    0000301511
    0000336165
    0000301511
    0000255943

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    Effect of culture conditions on microorganism identification by matrix-assisted laser desorption ionization mass spectrometry.
    Valentine N, et al.
    Applied and Environmental Microbiology, 71(1), 58-64 (2005)
    Identification and characterization of a two-component sensor-kinase and response-regulator system (DcuS-DcuR) controlling gene expression in response to C4-dicarboxylates in Escherichia coli.
    Golby P, et al.
    Journal of Bacteriology, 181(4), 1238-1248 (1999)
    Joshua W McCausland et al.
    Nature communications, 12(1), 609-609 (2021-01-29)
    The FtsZ protein is a central component of the bacterial cell division machinery. It polymerizes at mid-cell and recruits more than 30 proteins to assemble into a macromolecular complex to direct cell wall constriction. FtsZ polymers exhibit treadmilling dynamics, driving

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