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Safety Information

FLAGIPT1

Millipore

FLAG® Immunoprecipitation Kit

Synonym(s):

Anti-ddddk, Anti-dykddddk

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Select a Size

1 L
¥397.95
2.5 L
¥599.59
4 L
¥1,336.23
4 L
¥1,420.85
10 L
¥2,431.86
25 L
¥2,845.17

¥397.95

Estimated to ship on2025年4月22日Details

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1 L
¥397.95
2.5 L
¥599.59
4 L
¥1,336.23
4 L
¥1,420.85
10 L
¥2,431.86
25 L
¥2,845.17

About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

¥397.95

Estimated to ship on2025年4月22日Details

usage

 kit sufficient for 50 reactions

Quality Level

300

analyte chemical class(es)

proteins (FLAG® Affinity Gels)

technique(s)

immunoprecipitation (IP): suitable

shipped in

wet ice

storage temp.

−20°C

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assay range

accuracy: 76-103%, standard curve range: 2-200 μU/mL, linearity: 96-112%

assay range

accuracy: 88-103%, linearity: 84-120%, sensitivity: 0.5 pM, standard curve range: 2-200 pM

assay range

accuracy: 91-111%, linearity: 86-108%, standard curve range: 0.2-20 ng/mL

assay range

accuracy: 86.7%, linearity: 99.9%, sensitivity: 8.2 pg/mL, standard curve range: 8.2-2000 pg/mL

input

sample type plasma (K2 EDTA)
sample type serum
sample type cell culture supernatant

input

sample type serum
sample type plasma (K2 EDTA)

input

sample type plasma (K2 EDTA)
sample type serum

input

sample type cell culture supernatant
sample type serum
sample type plasma (K2 EDTA)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

detection method

colorimetric (450nm)

detection method

colorimetric (450nm)

detection method

colorimetric (450nm)

detection method

colorimetric (450nm/590nm)

General description

Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.

Application

Learn more product details in our FLAG® application portal.

Features and Benefits

  • Utilizes highly specific ANTI-FLAG M2 affinity gel
  • No preliminary steps or calibrations
  • 3xFLAG™ peptide provides efficient and gentle elution through direct competition

Other Notes

All prodedures to be performed at 2-8°C.

Legal Information

3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS
  • A2220ANTI-FLAG M2 affinity gel 1 mL
  • SAE01943X FLAG peptide 1 mg

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

Regulatory Information

常规特殊物品

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000162028
    0000162028
    0000162024
    0000162024
    0000121265

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    Find documentation for the products that you have recently purchased in the Document Library.

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