Key Documents

View All Documentation

D1946

DAF-FM DA

Name: DAF-FM DA
Brand: SIGMA

≥98% (HPLC), NO detector, film

Synonym(s):

4-Amino-5-(N-methylamino)-3′,6′-bis(acetyloxy)-2′,7′-difluoro-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, Diaminofluorescein-FM diacetate

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):

C₂₅H₁₈F₂N₂O₇

CAS Number:

254109-22-3

Molecular Weight:

496.42

UNSPSC Code:

12352200

PubChem Substance ID:

329798717

NACRES:

NA.77

MDL number:

MFCD16872050

Assay:

≥98% (HPLC)

Form:

powder

Quality level:

100

Storage condition:

desiccated

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

DAF-FM DA, ≥98% (HPLC)

Quality Level

100

assay

≥98% (HPLC)

form

powder

storage condition

desiccated

color

colorless to yellow

fluorescence

λ_ex 500 nm; λ_em 515 nm in DMSO

shipped in

wet ice

storage temp.

−20°C

SMILES string

CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(OC(C)=O)cc4Oc5cc(OC(C)=O)c(F)cc35

InChI

1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3

InChI key

BEVHTVRRVVEMEF-UHFFFAOYSA-N

Description

Application

DAF-FM DA has been used to measure the nitric oxide (NO) levels in liver tissue homogenates. It has also been used in NO staining for detection of NO from human umbilical vein endothelial cells (HUVECs).

Biochem/physiol Actions

DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO).

DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.

Features and Benefits

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Disclaimer

Hygroscopic

Still not finding the right product?

Explore all of our products under DAF-FM DA

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Nitric Oxide Synthases

We offer many products related to nitric oxide synthases for your research needs.

Sensor Dyes

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

DISCOVER Bioactive Small Molecules for Nitric Oxide & Cell Stress Research

View All Articles

Concentration-dependent osteogenic and angiogenic biological performances of calcium phosphate cement modified with copper ions

Zhang J, et al.

Materials Science and Engineering, C, 99, 1199-1212 (2019)

The role of nitric oxide in the interaction of Arabidopsis thaliana with the biotrophic fungi, Golovinomyces orontii and Erysiphe pisi.

Markus Schlicht et al.

Frontiers in plant science, 4, 351-351 (2013-09-24)

Powdery mildews are a diverse group of pathogenic fungi that can infect a large number of plant species, including many economically important crops. However, basic and applied research on these devastating diseases has been hampered by the obligate biotrophic lifestyle

Nitric oxide signaling is involved in the response to iron deficiency in the woody plant Malus xiaojinensis.

Longmei Zhai et al.

Plant physiology and biochemistry : PPB, 109, 515-524 (2016-11-12)

To cope with iron (Fe) deficiency, plants have evolved a wide range of adaptive responses from changes in morphology to altered physiological responses. Recent studies have demonstrated that nitric oxide (NO) is involved in the Fe-deficiency response through hormonal signaling

View All Related Papers

Global Trade Item Number

SKU	GTIN
D1946-1MG	04061833561133