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D1946

Sigma-Aldrich

DAF-FM DA

≥98% (HPLC)

Synonym(s):

4-Amino-5-(N-methylamino)-3′,6′-bis(acetyloxy)-2′,7′-difluoro-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, Diaminofluorescein-FM diacetate

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CN¥7,052.02

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1 MG
CN¥7,052.02

About This Item

Empirical Formula (Hill Notation):
C25H18F2N2O7
CAS Number:
254109-22-3
Molecular Weight:
496.42
MDL number:
MFCD16872050
UNSPSC Code:
12352200
PubChem Substance ID:
329798717
NACRES:
NA.77

CN¥7,052.02

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Quality Level

100

Assay

≥98% (HPLC)

form

film

storage condition

desiccated

color

colorless to yellow

fluorescence

λex 500 nm; λem 515 nm in DMSO

shipped in

wet ice

storage temp.

−20°C

SMILES string

CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(OC(C)=O)cc4Oc5cc(OC(C)=O)c(F)cc35

InChI

1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3

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Show Differences

1 of 4

This Item
D1821D2196D2321
DAF-FM DA ≥98% (HPLC)

D1946

DAF-FM DA

DAF-FM ≥98% (HPLC)

D1821

DAF-FM

DAF-FM solution 5 mM in DMSO, ≥97% (HPLC)

D2196

DAF-FM solution

DAF-FM DA solution 5 mM in DMSO, ≥97% (HPLC)

D2321

DAF-FM DA solution

form

film

form

powder

form

DMSO solution

form

DMSO solution

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage condition

desiccated

storage condition

desiccated

storage condition

desiccated

storage condition

desiccated, protect from light

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

Application

DAF-FM DA has been used to measure the nitric oxide (NO) levels in liver tissue homogenates.[1] It has also been used in NO staining for detection of NO from human umbilical vein endothelial cells (HUVECs).[2][3]

Biochem/physiol Actions

DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO).
DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.

Features and Benefits

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Caution

Hygroscopic

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Lot/Batch Number
    0000073468
    0000086670
    0000074015
    0000044855
    0000044237

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    Markus Schlicht et al.
    Frontiers in plant science, 4, 351-351 (2013-09-24)
    Powdery mildews are a diverse group of pathogenic fungi that can infect a large number of plant species, including many economically important crops. However, basic and applied research on these devastating diseases has been hampered by the obligate biotrophic lifestyle
    Concentration-dependent osteogenic and angiogenic biological performances of calcium phosphate cement modified with copper ions
    Zhang J, et al.
    Materials Science and Engineering, C, 99, 1199-1212 (2019)
    Bing Fang Luo et al.
    Journal of experimental botany, 63(8), 3127-3136 (2012-03-02)
    Nitrogen (N) management is a promising agronomic strategy to minimize cadmium (Cd) contamination in crops. However, it is unclear how N affects Cd uptake by plants. Wild-type and iron uptake-inefficient tomato (Solanum lycopersicum) mutant (T3238fer) plants were grown in pH-buffered
    Longmei Zhai et al.
    Plant physiology and biochemistry : PPB, 109, 515-524 (2016-11-12)
    To cope with iron (Fe) deficiency, plants have evolved a wide range of adaptive responses from changes in morphology to altered physiological responses. Recent studies have demonstrated that nitric oxide (NO) is involved in the Fe-deficiency response through hormonal signaling
    Shaoting Du et al.
    Journal of experimental botany, 67(3), 893-904 (2015-11-27)
    CO2 elevation often alters the plant's nitrate reductase (NR) activity, the first enzyme acting in the nitrate assimilation pathway. However, the mechanism underlying this process remains unknown. The association between elevated CO2-induced alterations of NR activity and nitric oxide (NO)
    View All Related Papers

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