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Merck
CN

251500-M

DAF-2

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

Synonym(s):

DAF-2, 4,5-Diaminofluorescein

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About This Item

Empirical Formula (Hill Notation):
C20H14N2O5
CAS Number:
205391-01-1
Molecular Weight:
362.34
UNSPSC Code:
12171500
NACRES:
NA.25
MDL number:
MFCD05865100

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Product Name

DAF-2, A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

SMILES string

Nc1cc2c(cc1N)C(=O)OC32c4c(cc(cc4)O)Oc5c3ccc(c5)O

InChI

1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2

InChI key

LTYUPYUWXRTNFQ-UHFFFAOYSA-N

assay

≥98% (HPLC)

form

solution

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

pale yellow

fluorescence

λex ~495 nm
λem ~515 nm

storage temp.

−20°C

Quality Level

100

Biochem/physiol Actions

Primary Target
Fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

Disclaimer

Toxicity: Irritant (B)

General description

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vivo. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent compound triazolofluorescein (DAF-2 T). Can detect NO under neutral conditions (detection limit = 5 nM). Excitation maximum: 495 nm; emission maximum: 515 nm.

Legal Information

Manufactured by Daiichi Pure Chemicals Co., Ltd
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Berkels, R., et al. 2000. Cell Calcium27, 281.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.

Physical form

A 5 mM (1 mg in 550 µl) solution in DMSO.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

188.6 °F - closed cup - (Dimethylsulfoxide)

flash_point_c

87 °C - closed cup - (Dimethylsulfoxide)

Certificates of Analysis (COA)

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