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Merck
CN

251515-M

DAF-FM

A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.

Synonym(s):

DAF-FM, 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein

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About This Item

Empirical Formula (Hill Notation):
C21H14F2N2O5
CAS Number:
254109-20-1
Molecular Weight:
412.34
UNSPSC Code:
12352200

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Product Name

DAF-FM, A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.

InChI key

DIJCILWNOLHJCG-UHFFFAOYSA-N

InChI

1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3

assay

≥98% (HPLC)

form

solution

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

pale yellow

fluorescence

λex ~500 nm
λem ~515 nm

storage temp.

−20°C

Quality Level

100

Biochem/physiol Actions

Primary Target
Nitric oxide (NO) fluorescent indicator

Disclaimer

Toxicity: Irritant (B)

General description

A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.

Legal Information

Manufactured by Daiichi Pure Chemicals Co., Ltd
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew. Chem. Int. Ed.38, 3209.

Physical form

In DMSO.

Preparation Note

Following initial thaw, aliquot and freeze at -20°C. Use immediately after dilution, discard any unused portion.

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

188.6 °F

flash_point_c

87 °C

Certificates of Analysis (COA)

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