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Merck
CN

C8982

Sigma-Aldrich

α-Cyano-4-hydroxycinnamic acid

suitable for MALDI-TOF MS

Synonym(s):

α-CCA, α-CHCA, α-Cyano, 4-HCCA, ACCA

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1 MG
CN¥669.75
5 MG
CN¥2,560.22
10 MG
CN¥4,050.78

CN¥669.75


Estimated to ship onJuly 02, 2025Details

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1 MG
CN¥669.75
5 MG
CN¥2,560.22
10 MG
CN¥4,050.78

About This Item

Linear Formula:
HOC6H4CH=C(CN)CO2H
CAS Number:
Molecular Weight:
189.17
Beilstein:
3271427
EC Number:
MDL number:
UNSPSC Code:
12352106
PubChem Substance ID:
NACRES:
NA.21

CN¥669.75


Estimated to ship onJuly 02, 2025Details

New, lower price on this item!

Request a Bulk Order

form

powder

analyte chemical class(es)

glycans, peptides, proteins

mp

245-250 °C (lit.)

suitability

suitable for MALDI-TOF MS

storage temp.

room temp

SMILES string

OC(=O)\C(=C\c1ccc(O)cc1)C#N

InChI

1S/C10H7NO3/c11-6-8(10(13)14)5-7-1-3-9(12)4-2-7/h1-5,12H,(H,13,14)/b8-5+

InChI key

AFVLVVWMAFSXCK-VMPITWQZSA-N

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This Item
171006117100981710044
species reactivity

human, mouse

species reactivity

human, Xenopus

species reactivity

human, zebrafish, mouse, Xenopus, chicken, fish

species reactivity

human

clone

monoclonal

clone

monoclonal

clone

monoclonal

clone

1.2.1.5.4, monoclonal

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

technique(s)

ChIP: suitable, immunoprecipitation (IP): suitable, dot blot: suitable, western blot: suitable

technique(s)

ChIP: suitable (ChIP-seq), immunoprecipitation (IP): suitable

technique(s)

ChIP: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ChIP: suitable, ELISA: suitable, dot blot: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, multiplexing: suitable, western blot: suitable

Gene Information

human ... TCF7L2(6934)

Gene Information

-

Gene Information

human ... TBP(6908)

Gene Information

human ... CTCF(10664)

General description

α-Cyano-4-hydroxycinnamic acid (CHCA) is specially purified and qualified as a matrix for peptide MALDI-MS (matrix assisted laser desorption ionization-mass spectrometry) calibration standards. CHCA enhances ionization of protein or peptide standards and samples in matrix assisted laser desorption ionization (MALDI) mass spectrometers.[1][2]
It is also available as part of our ProteoMass MALDI calibration kits (MS-CAL1 and MS-CAL3).

Application

α-Cyano-4-hydroxycinnamic acid has been used as a matrix for MALDI-TOF MS (matrix assisted laser desorption ionization-time of flight mass spectrometry) analysis. The matrix has also been used for sample preparation.[3][4][5]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Legal Information

ProteoMass is a trademark of Sigma-Aldrich Co. LLC

related product

Product No.
Description
Pricing

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1B

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What can be used for solublization of α-Cyano-4-hydroxycinnamic acid?

    α-Cyano-4-hydroxycinnamic acid (α-CCA, α-CHCA, α-Cyano, 4-HCCA, ACCA) is soluble in methanol (up to 50 mg/ml). It is also soluble at 10 mg/ml using 50% acetonitrile in 0.05% TFA for MALDI-MS. The acetonitrile concentration can be adjusted for individual preferences.For biological applications, it has been solubilized at 100 mM in DMSO or 50 mM in ethanol.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

a-Cyano-4-hydroxycinnamic acid as a matrix for matrixassisted laser desorption mass spectromtry.
Beavis RC, et al.
Journal of Mass Spectrometry : Jms, 27, 156-158 (1992)
Utilizing isotope dilution-matrix-assisted laser desorption ionization-time of flight mass spectrometry as a reference procedure for the radioimmunoassay of serum thyroxine.
Tyan YC, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 420, 99-103 (2013)
MALDI-TOF MS sample preparation by using alkanethiolate self-assembled monolayers: A preliminary application for protein sample analysis.
Tyan Y-C, et al.
International Journal of Mass Spectrometry, 262, 67-72 (2007)
Jarod A Fincher et al.
The Analyst, 145(21), 6910-6918 (2020-08-26)
Mass spectrometry imaging (MSI) is a powerful analytical technique that enables detection, discovery, and identification of multiple classes of biomolecules, while simultaneously mapping their spatial distributions within a sample (e.g., a section of biological tissue). The limitation in molecular coverage
J Gobom et al.
Analytical chemistry, 73(3), 434-438 (2001-02-24)
We present a new MALD1 sample preparation technique for peptide analysis using the matrix alpha-cyano-4-hydroxy-cinnamic acid (CHCA) and prestructured sample supports. The preparation integrates sample purification, based on the affinity of microcrystalline CHCA for peptides, thereby simplifying the analysis of

Protocols

Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.

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