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B6650

Sigma-Aldrich

X-GlcA

reagent for selection of recombinant bacterial clones

Synonym(s):

5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, X-GlcA, X-glucuronide CHA salt

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10 MG
¥1,617.22
25 MG
¥2,669.81
100 MG
¥8,019.88

¥1,617.22

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10 MG
¥1,617.22
25 MG
¥2,669.81
100 MG
¥8,019.88

About This Item

Empirical Formula (Hill Notation):
C14H13BrClNO7 · C6H13N
CAS Number:
114162-64-0
Molecular Weight:
521.79
MDL number:
MFCD00058543
UNSPSC Code:
12352200
PubChem Substance ID:
24891926
NACRES:
NA.85

¥1,617.22

Please contact Customer Service for Availability
New, lower price on this item!

grade

Molecular Biology

Quality Level

200

sterility

non-sterile

Assay

≥98% (TLC)

form

powder

technique(s)

nucleic acid detection: suitable

solubility

DMF: soluble

suitability

suitable for β-galactosidase test

storage temp.

−20°C

SMILES string

NC1CCCCC1.O[C@@H]2[C@@H](O)[C@@H](O[C@@H]([C@H]2O)C(O)=O)Oc3c[nH]c4ccc(Br)c(Cl)c34

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1 of 4

This Item
B9146B2904B6024
X-GlcA reagent for selection of recombinant bacterial clones

B6650

X-GlcA

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷 ≥98%

B9146

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷

卤代腺苷基-beta-D-半乳糖苷 reagent for selection of recombinant bacterial clones

B2904

卤代腺苷基-beta-D-半乳糖苷

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷 tablet

B6024

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷

technique(s)

nucleic acid detection: suitable

technique(s)

-

technique(s)

nucleic acid detection: suitable

technique(s)

-

suitability

suitable for β-galactosidase test

suitability

suitable for identification of lac+ bacterial colonies

suitability

suitable for substrate for β-galactosidase

suitability

suitable for identification of lac+ bacterial colonies

form

powder

form

powder

form

powder

form

tablet

solubility

DMF: soluble

solubility

DMF: methanol (1:1): soluble 50 mg/mL

solubility

DMF: soluble

solubility

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Chromogenic substrate for β-glucuronidase (GUS) gene detection.
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.
X-GlcA has been used in histochemical staining of root sections for light microscopic observation.[1]

Principle

When X-GlcA is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GlcA turn an intense blue color, allowing for easy differentiation between lac+ and lac- colonies.
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    Stéphane Compant et al.
    Applied and environmental microbiology, 71(4), 1685-1693 (2005-04-07)
    Patterns of colonization of Vitis vinifera L. cv. Chardonnay plantlets by a plant growth-promoting bacterium, Burkholderia sp. strain PsJN, were studied under gnotobiotic conditions. Wild-type strain PsJN and genetically engineered derivatives of this strain tagged with gfp (PsJN::gfp2x) or gusA
    Differential colonization of tomato roots by nonpathogenic and pathogenic Fusarium oxysporum strains may influence Fusarium wilt control
    Bao J R and Lazarovits G
    Phytopathology, 91(5), 449-456 (2001)
    Li Liu et al.
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 21(9), 2014-2019 (2007-03-14)
    Gene therapy shows promise for treating prostate cancer and has been evaluated in several clinical trials. A major challenge that remains is to establish a method for verifying transgene activity in situ. The lacZ gene encoding beta-galactosidase historically has been
    Quoc Phong Le et al.
    Foodborne pathogens and disease, 12(8), 719-725 (2015-06-26)
    Contamination of food with multiantibiotic-resistant bacteria, particularly extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae, is considered a potential source for the wide dissemination of ESBL-producing bacteria in communities. However, little is known about the extent of contamination of food with ESBL-producing bacteria in
    S K Kritas et al.
    Journal of animal science, 93(1), 405-413 (2015-01-09)
    This field study assessed the efficacy of a probiotic based on viable spores of Bacillus subtilis C-3102 (Calsporin; Calpis Co. Ltd., Japan) on the health status and productivity of sows and their litters through 2 full, sequential reproductive cycles from
    View All Related Papers

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