CN¥3,832.67
国内现货，预计发货时间April 25, 2025详情
推荐产品
等级
Molecular Biology
质量水平
无菌性
non-sterile
表单
powder
技术
microbiological culture: suitable
适用性
suitable for β-galactosidase test
nonselective for Escherichia coli
nonselective for coliforms
应用
food and beverages
microbiology
储存温度
room temp
1 of 4
此商品 | 17409 | 17408 | 17245 |
|---|---|---|---|
| technique(s) immunoprecipitation (IP): suitable | technique(s) immunoprecipitation (IP): suitable | technique(s) immunoprecipitation (IP): suitable | technique(s) immunoprecipitation (IP): suitable |
| manufacturer/tradename EZ-ChIP | manufacturer/tradename Magna ChIP® | manufacturer/tradename Magna ChIP® | manufacturer/tradename Upstate® |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| shipped in wet ice | shipped in dry ice | shipped in dry ice | shipped in - |
一般描述
S-Gal® (sodium salt) is an autoclavable, water-soluble, chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. S-gal® is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
应用
S-Gal®/LB Agar Blend has been used for the identification of recombinant bacterial colonies.[1][2][3]
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. S-Gal® (sodium salt) is water-soluble, autoclavable and can be added to bacterial broth containing agar prior to autoclaving.
特点和优势
- More intense color contrast than X-gal
- Water-soluble and autoclavable for easiest use
- Convenient, pre-mixed media
组分
Ingredients (g/L)
Tryptone, 10
Yeast extract, 5
Sodium chloride, 10
Agar, 12
S-Gal, 0.3
Ferric ammonium citrate, 0.5
IPTG, 0.03
Tryptone, 10
Yeast extract, 5
Sodium chloride, 10
Agar, 12
S-Gal, 0.3
Ferric ammonium citrate, 0.5
IPTG, 0.03
原理
When S-Gal® is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal® and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies.
制备说明
Suspend contents of one packet in 500 ml distilled or deionized water. Sterilize by autoclaving for 15 to 20 minutes at 121-124°C. For microwaving, heat suspended mix until initial boiling. Mix well. Heat for short intervals with mixing until agar component is in solution. Do not allow boiling for extended periods of time. Antibiotics should be added following autoclaving or microwaving, after cooling to 48-52°C.
其他说明
The ferric or Fe3+ ion is required for color development and must be added to any S-Gal®
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.
法律信息
S-GAL is a registered trademark of Merck KGaA, Darmstadt, Germany
相关产品
产品编号
说明
价格
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
LifeScience Quarterly: A superior dye to X-gal for clonal selection.
Heuermann, K. and Cosgrove, J.
Sigma data, 2(2), 2-4 (2001)
Paola Jurado et al.
Molecular microbiology, 60(5), 1218-1227 (2006-05-13)
The identification of single-chain antibodies (scFvs) that interfere in vivo with the building of the complex that activate the prokaryotic, sigma54-dependent promoter Pu of the catabolic TOL plasmid pWW0 is reported. To this end, a phage M13 library of scFvs
John Kelly et al.
Journal of bacteriology, 188(7), 2427-2434 (2006-03-21)
In eukaryotes, N-linked protein glycosylation is a universal modification involving addition of preformed oligosaccharides to select Asn-Xaa-Ser/Thr motifs and influencing multiple biological events. We recently demonstrated that Campylobacter jejuni is the first member of the Bacteria to possess an N-linked
Heat shock protein 70 (Hsp70) subtype expression in neuroendocrine tissue and identification of a neuroendocrine tumour-specific Hsp70 truncation.
Zierhut B, et al.
Endocrine-related cancer, 11(2), 377-389 (2004)
N Martin Young et al.
The Journal of biological chemistry, 277(45), 42530-42539 (2002-08-21)
Mass spectrometry investigations of partially purified Campylobacter jejuni protein PEB3 showed it to be partially modified with an Asn-linked glycan with a mass of 1406 Da and composed of one hexose, five N-acetylhexosamines and a species of mass 228 Da
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