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Safety Information

B8931

Sigma-Aldrich

Magenta-Gal

reagent for selection of recombinant bacterial clones

Synonym(s):

Red-Gal, 5-Bromo-6-chloro-3-indolyl β-D-galactopyranoside

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100 EA
CN¥800.36

CN¥800.36

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100 EA
CN¥800.36

About This Item

Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
93863-88-8
Molecular Weight:
408.63
MDL number:
MFCD02683897
UNSPSC Code:
12352200
PubChem Substance ID:
329773145
NACRES:
NA.85

CN¥800.36

Please contact Customer Service for Availability

grade

Molecular Biology

Quality Level

200

sterility

non-sterile

product line

BioReagent

Assay

≥98.0% (HPLC)

form

powder

solubility

methanol: soluble

suitability

suitable for substrate for β-galactosidase

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O

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HPTLC plates, Silica gel 60 F₂₅₄ pkg of 50 plates, plate L × W 20 cm × 10 cm, glass support

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layer thickness

200 μm

layer thickness

200 μm

layer thickness

200 μm

layer thickness

200 μm

feature

binder Organic Polymer, fluorescent indicator

feature

binder Organic Polymer, fluorescent indicator: no

feature

binder Organic Polymer, fluorescent indicator

feature

binder Organic Polymer, fluorescent indicator

technique(s)

thin layer chromatography (TLC): suitable

technique(s)

thin layer chromatography (TLC): suitable

technique(s)

thin layer chromatography (TLC): suitable

technique(s)

thin layer chromatography (TLC): suitable

packaging

pkg of 25 plates

packaging

pkg of 25 plates

packaging

pkg of 25 sheets

packaging

pkg of 50 plates

plate L × W

10 cm × 20 cm

plate L × W

20 cm × 20 cm

plate L × W

-

plate L × W

20 cm × 10 cm

General description

Red-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Red-Gal is a chromogenic substrate for b-galactosidase,[1] used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in the blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Chromogenic substrate for β-galactosidase producing a red or magenta insoluble product.
Magenta-Gal has been used for staining synthetic cytoplasmic or nuclear LacZ mRNA.[2] It has also been used to detect the expression of the EphB1-β-gal fusion protein.[1] ,Magenta-Gal is suitable for use in the selection of recombinant bacterial colonies with the lac+ phenotype.

Principle

When Red-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Red-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCJ9184
    BCBK8608V
    BCBJ5700V
    1403239V
    1403239

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    Nature communications, 6, 7476-7476 (2015-06-24)
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    Members of the fibroblast growth factor (FGF) family play important roles during various developmental processes including eye development. FRS (FGF receptor substrate) proteins bind to FGFR and serve as adapters for coordinated assembly of multi-protein complexes involved in Ras/MAPK and

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