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Merck
CN

16669

Magenta-Gal

reagent for selection of recombinant bacterial clones

Synonym(s):

5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside, Mag-Gal

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About This Item

Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
93863-88-8
Molecular Weight:
408.63
Beilstein:
1552602
MDL number:
MFCD00210022
UNSPSC Code:
12352200
PubChem Substance ID:
329751317

Key Documents

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grade

Molecular Biology

Quality Level

100

sterility

non-sterile

Assay

≥98.0% (HPLC)

form

powder

solubility

DMF: soluble
DMSO: soluble

suitability

suitable for β-galactosidase test

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11-,12+,13-,14-/m1/s1

InChI key

CHRVKCMQIZYLNM-RKQHYHRCSA-N

General description

Magenta-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Magenta-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Also suitable for use immunoblotting and immunocytochemical assays.

Biochem/physiol Actions

When Magenta-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Magenta-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Yeon-Jin Kim et al.
Developmental biology, 397(1), 129-139 (2014-12-03)
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