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Safety Information

B2904

Sigma-Aldrich

Bluo-Gal

reagent for selection of recombinant bacterial clones

Synonym(s):

5-Bromo-3-indolyl β-D-galactopyranoside, substrate for β-galactosidase, Bluo-Gal

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About This Item

Empirical Formula (Hill Notation):
C14H16BrNO6
CAS Number:
97753-82-7
Molecular Weight:
374.18
Beilstein:
1550140
MDL number:
MFCD00063691
UNSPSC Code:
12352200
PubChem Substance ID:
24891417
NACRES:
NA.85

grade

Molecular Biology

Quality Level

200

sterility

non-sterile

Assay

≥98% (HPLC)

form

powder

technique(s)

nucleic acid detection: suitable

solubility

DMF: soluble

suitability

suitable for substrate for β-galactosidase

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O

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A4503

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A3912

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牛血清白蛋白 来源于牛血清 heat shock fraction, pH 5.2, ≥96%

A8022

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A9647

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assay

≥96%

assay

≥96%

assay

≥96%

assay

≥98%

biological source

bovine

biological source

bovine

biological source

bovine

biological source

bovine

origin

USA origin

origin

USA origin

origin

USA origin

origin

USA origin

Quality Level

300

Quality Level

200

Quality Level

300

Quality Level

300

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

General description

Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis,[1] Dictyostelium samples[2] and in the staining of vena cava of adult mice.[3]
Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.

Principle

When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

监管及禁止进口产品
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    Lot/Batch Number
    0000434036
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    0000396640
    0000396641
    0000387702

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    Leslie M Stone et al.
    The Journal of neuroscience : the official journal of the Society for Neuroscience, 22(11), 4522-4529 (2002-06-01)
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    List K, et al.
    The American Journal of Pathology, 168(5), 1513-1525 (2006)
    Ammonium transporter C of Dictyostelium discoideum is required for correct prestalk gene expression and for regulating the choice between slug migration and culmination
    Kirsten JH, et al.
    Developmental Biology, 287(1), 146-156 (2005)
    Kyoji Horie et al.
    Molecular and cellular biology, 23(24), 9189-9207 (2003-12-03)
    The use of mutant mice plays a pivotal role in determining the function of genes, and the recently reported germ line transposition of the Sleeping Beauty (SB) transposon would provide a novel system to facilitate this approach. In this study
    H T Yuan et al.
    Journal of the American Society of Nephrology : JASN, 11(6), 1055-1066 (2000-05-23)
    Angiopoietin-1 (Ang-1) stimulates endothelial and vascular network differentiation through the Tie-2 receptor tyrosine kinase, while Ang-2 modulates this activation in embryo and tumor growth. The nephrogenic pattern of Ang-2 was documented in a mouse strain that expresses the LacZ reporter
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