Skip to Content
Merck
CN
All Photos(3)

Documents

Safety Information

B9146

Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

≥98%

Sign Into View Organizational & Contract Pricing
All Photos(3)
Synonym(s):
5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal
Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
7240-90-6
Molecular Weight:
408.63
Beilstein:
1402009
EC Number:
230-640-8
MDL number:
MFCD00005666
UNSPSC Code:
12352204
PubChem Substance ID:
57653968
NACRES:
NA.83

Quality Level

200

Assay

≥98%

form

powder

solubility

DMF: methanol (1:1): soluble 50 mg/mL

suitability

suitable for identification of lac+ bacterial colonies

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI key

OPIFSICVWOWJMJ-AEOCFKNESA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside has been used to confirm transduction of Caki-2/LacZ clones. It has also been used in blue-white selection of transformants.
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

Reconstitution

A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20 °C and protected from light. Sterilization is not required.

related product

Product No.
Description
Pricing

94433

β-Galactosidase stain

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

监管及禁止进口产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

HSV-tk gene therapy for human renal cell carcinoma in nude mice
Pulkkanen K, et al.
Cancer Gene Therapy, 8(7), 529-529 (2001)
Rolling circle amplification to screen yam germplasm for badnavirus infections and to amplify and characterise novel badnavirus genomes
Bomer M, et al.
Bio-protocol, 8(1), 529-529 (2018)
Enzo R Porrello et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(1), 187-192 (2012-12-19)
We recently identified a brief time period during postnatal development when the mammalian heart retains significant regenerative potential after amputation of the ventricular apex. However, one major unresolved question is whether the neonatal mouse heart can also regenerate in response
Khanh-Van Tran et al.
Cell metabolism, 15(2), 222-229 (2012-02-14)
Adipose tissue expansion involves the enlargement of existing adipocytes, the formation of new cells from committed preadipocytes, and the coordinated development of the tissue vascular network. Here we find that murine endothelial cells (ECs) of classic white and brown fat
Yibing Wang et al.
PloS one, 8(3), e59195-e59195 (2013-03-26)
Our study had three objectives: to extend the plasmid-based transformation protocol to a clinical isolate of C. trachomatis belonging to the trachoma biovar, to provide "proof of principle" that it is possible to "knock out" selected plasmid genes (retaining a
View All Related Papers

Articles

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

Yeast Transformation Introduction

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

Protocols

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service