B9146
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside
≥98%, powder, suitable for identification of lac+ bacterial colonies
Synonym(s):
5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal
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About This Item
Product Name
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, ≥98%
Quality Level
Assay
≥98%
form
powder
solubility
DMF: methanol (1:1): soluble 50 mg/mL
suitability
suitable for identification of lac+ bacterial colonies
storage temp.
−20°C
SMILES string
OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O
InChI
1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
InChI key
OPIFSICVWOWJMJ-AEOCFKNESA-N
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Related Categories
Application
Preparation Note
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Regulatory Information
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Articles
General protocols for growth of competent cells and their transformation (uptake of DNA).
Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.
感受态细胞生长及其转化（吸收DNA）的通用实验方案。
转化是将外源DNA引入细胞，引起遗传变异或基因修饰的过程。1928年，Griffith首次报道了利用肺炎链球菌进行的转化。Avery等人于1944年证明了DNA转化的原理。
Protocols
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.
Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.
General protocols for growth of competent cells in microbial medium.
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
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