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Key Documents

Safety Information

A9647

Sigma-Aldrich

Bovine Serum Albumin

heat shock fraction, pH 7, ≥98%

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

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10 MG
¥416.52
25 G
¥664.50
100 G
¥897.36
1 KG
¥4,486.61

¥416.52


Available to ship on2025年4月10日Details


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10 MG
¥416.52
25 G
¥664.50
100 G
¥897.36
1 KG
¥4,486.61

About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.27

¥416.52


Available to ship on2025年4月10日Details


Request a Bulk Order

biological source

bovine

Assay

≥98%

form

lyophilized powder

mol wt

~66 kDa

purified by

heat shock fractionation

origin

USA origin

technique(s)

immunocytochemistry: suitable

impurities

≤5.0% Loss on drying

loss

≤5%

pH

7

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This Item
546585554995653438
mol wt

Mw ≥20000

mol wt

-

mol wt

-

mol wt

-

solubility

chloroform: soluble

solubility

chloroform: soluble

solubility

chlorinated solvents: soluble (including chloroform)

solubility

-

λmax

365 nm

λmax

-

λmax

381 nm

λmax

-

fluorescence

λex 374 nm; λem 417 nm in chloroform(lit.)

fluorescence

λex 387 nm; λem 420 nm in chloroform

fluorescence

λex 383 nm; λem 419 nm in chloroform

fluorescence

λex 406 nm; λem 490 nm in chloroform

OLED device performance

ITO/PEDOT:PSS/PVK/PFO/Ca

  • Color: blue
  • Max. Luminance: 102.3 Cd/m2
  • Max. EQE: 0.17 %

OLED device performance

-

OLED device performance

-

OLED device performance

-

General description

Bovine serum albumin is a protein whose key biological function is to regulate the colloidal osmotic pressure of blood. BSA is widely used in various studies and applications. Made up of 583 amino acids,[1][2] BSA is a water-soluble protein with a calculated molecular weight of 66,430 Da. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.[3]

Application

Bovine Serum Albumin is used for many applications, including the following applications:
  • BSA has been used in cell culture, e.g. as a supplement in media prepared for culturing of oligodendrocytes.[4]
  • BSA has been used for the dilution of primary antibody solution in immunohistochemistry procedures.[5]
  • BSA has been used as a standard in the Bradford method of protein quantification.[6]
  • BSA has been used in blocking and permeabilization buffer for immunofluorescence studies.[7]

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

新产品

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the molecular weight of Product A9647, Albumin from bovine serum?

    The molecular weight of BSA is 66,430.

  4. How can the concentration of a solution of Product A9647, Albumin from bovine serum, be quantified?

    The concentration of a solution of BSA can be measured by using the extinction coefficient (E1%) of 6.67. The 1% refers to a solution that is 1% weight-to-volume (i.e., 10 mg/mL).

  5. What is the pH of a solution of Product A9647, Albumin from bovine serum?

    The pH of a 1% solution is approximately 7.0, according to the product specifications.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Characterization of an Exo-beta-1,3-Glucanase Produced by Pichia anomala Strain K, Antagonist of Botrytis cinerea on Apples.
Jijakli MH et al.
Phytopathology, 88, 335-335 (1998)
E-cadherin, MMP-2, and MMP-9 as prognostic markers in penile cancer: analysis of 125 patients.
Campos RS et al.
Urology, 67, 797-797 (2006)
Down-regulation of seladin-1 increases BACE1 levels and activity through enhanced GGA3 depletion during apoptosis.
Sarajarvi T et al.
The Journal of Biological Chemistry, 284, 34433-34433 (2009)
Mohammad Bahrami et al.
Scientific reports, 9(1), 11739-11739 (2019-08-15)
Defining oocyte in vitro maturation (IVM) conditions allows for improved reproducibility and efficiency of bovine embryo production. IVM conditions for bovine oocytes have been extensively studied, but beneficial effects of individual supplements remain controversial. This study compared methods of cumulus
Corrigendum: Formation and reshuffling of disulfide bonds in bovine serum albumin demonstrated using tandem mass spectrometry with collision-induced and electron-transfer dissociation.
Rombouts I et al.
Scientific Reports, 5 (2015)

Articles

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.

Explore serum albumin's role in serum-free cell culture systems for biomanufacturing.

Protocols

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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