生物来源
bovine
质量水平
检测方案
≥98%
形式
lyophilized powder
分子量
~66 kDa
纯化方式
heat shock fractionation
包装
poly bottle of
来源
USA origin
技术
ELISA: suitable
blood typing: suitable
microbiological culture: suitable
杂质
protease, none detected
缺失
≤5%
pH值（酸碱度）
7
溶解性
water: soluble (40 mg/ml)
UniProt登记号
异质活性
BT Virus, none detected
VSV Virus, none detected
储存温度
2-8°C
基因信息
bovine ... ALB(280717)
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一般描述
白蛋白大量存在于人血浆中。白蛋白浓度升高会造成脱水。患有缺铁性贫血的儿童常见有白蛋白周转异常。可作为肝病的预测性指标。
应用
牛血清白蛋白已用于：
- 封闭抗体的非特异性结合
- 作为精子活力激发培养基组分
- 通过二辛可宁酸(BCA) 方法测定细菌素的蛋白浓度
生化/生理作用
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
特点和优势
- 热休克分级
- 无蛋白酶
制备说明
热激分级分离法制取
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
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相关内容
Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.
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