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A4955

Sigma-Aldrich

Alkaline Phosphatase Stabilizing Buffer

liquid

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100 G
CN¥980.32
500 G
CN¥881.77
1 KG
CN¥1,593.12

CN¥980.32

Available to ship onApril 10, 2025Details

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100 G
CN¥980.32
500 G
CN¥881.77
1 KG
CN¥1,593.12

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

CN¥980.32

Available to ship onApril 10, 2025Details

Request a Bulk Order

sterility

non-sterile

form

liquid

technique(s)

ELISA: suitable

storage temp.

2-8°C

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This Item
E5134ED2SS27285
乙二胺四乙酸 二钠盐 二水合物 Sigma Grade, suitable for plant cell culture, 98.5-101.5%

E6635

乙二胺四乙酸 二钠盐 二水合物

乙二胺四乙酸 二钠盐 二水合物 suitable for electrophoresis, for molecular biology, 99.0-101.0% (titration)

E5134

乙二胺四乙酸 二钠盐 二水合物

乙二胺四乙酸 二钠盐 二水合物 reagent grade, 98.5-101.5% (titration)

ED2SS

乙二胺四乙酸 二钠盐 二水合物

乙二胺四乙酸 二钠盐 二水合物 meets analytical specification of Ph. Eur., BP, USP, FCC, 99.0-101.0%

27285

乙二胺四乙酸 二钠盐 二水合物

grade

Sigma Grade

grade

for molecular biology

grade

reagent grade

grade

-

assay

98.5-101.5%

assay

99.0-101.0% (titration)

assay

98.5-101.5% (titration)

assay

99.0-101.0%

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

mp

248 °C (dec.) (lit.)

mp

248 °C (dec.) (lit.)

mp

248 °C (dec.) (lit.)

mp

248 °C (dec.) (lit.)

solubility

H2O: soluble, clear, colorless

solubility

3  M NaOH: 1.6 g/10 mL

solubility

H2O: soluble, clear, colorless

solubility

water: soluble 100 g/L at 20 °C

General description

Alkaline Phosphatase Stabilizing Buffer can extend the shelf life of alkaline phosphate conjugates.

Application

Alkaline Phosphatase (ALP) Stabilizing Buffer may be used in ALP assay in osteoblast cell protein lysates.[1][2] It may also be used as a component of antibody-enzyme conjugate dialysis buffer.[3]
Alkaline Phosphatase Stabilizing Buffer has been tested in ELISA systems. The product has been specially made for the stable dilution of alkaline phosphatase-antibody conjugates.It can be used for a series of experiments to achieve consistent results.

Components

Stabilizing compounds in Tris buffered saline, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

The degree of stability realized is highly dependent upon the conjugate tested. Trials using each particular conjugate should be performed to determine the degree of stability that can be expected. It is recommended that this buffer constitute ≥95% of the final solution.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

含少量动物源组分生物产品
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    Lot/Batch Number
    0000353750
    0000341764
    0000341765
    0000341765
    0000341764

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    ORegan T, et al.
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    Mesenchymal stem cells (MSCs) are multipotent cells, which can give rise to variety of cell types, including adipocytes and osteoblasts. Previously, we have shown that cysteine dioxygenase type 1 (Cdo1) promoted adipogenesis of primary mouse bone marrow stromal cells (BMSCs)
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