Skip to Content
Merck
CN
All Photos(1)

Documents

A9226

Sigma-Aldrich

Alkaline buffer solution

1.5 M, pH 10.3 (25 °C)

Synonym(s):

Basic pH buffer

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

form

liquid

Quality Level

concentration

1.5 M

pH

10.3 (25 °C)

storage temp.

2-8°C

Related Categories

General description

Alkaline buffer solution, 1.5 M, pH 10.3 at 25 °C is used in alkaline phosphatase reactions. It contains 2-amino-2-methylpropanol (AMP) and is used in conjunction with p-nitrophenyl phosphate (pNPP) enzyme substrate.

Application

Alkaline buffer solution has been used to measure the alkaline phosphatase (ALP) activity in the:
  • rat bone marrow stromal cells (BMSC)
  • osteoblast-like cells (SaoS-2, ATCC HTB 85)
  • mouse MC3T3-E1

Other Notes

Contains 2-amino-2-methylpropanol

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Young-Hee Kim et al.
BMC pharmacology & toxicology, 14, 60-60 (2013-12-03)
Indoxyl sulfate (IS), an organic anion uremic toxin, promotes the progression of renal dysfunction. Some studies have suggested that IS inhibits osteoclast differentiation and suppresses parathyroid hormone (PTH)-stimulated intracellular cAMP production, decreases PTH receptor expression, and induces oxidative stress in
Pin Huang et al.
Colloids and surfaces. B, Biointerfaces, 160, 628-638 (2017-10-17)
Increased use of reconstruction procedures in orthopedics has improved the life of patients undergoing surgery. However, surgical site infection remains a major challenge. Efforts were made to fabricate antibacterial surfaces with good biocompatibility. This present study aimed to fabricate zinc-incorporated
Salima Nedjari et al.
Scientific reports, 7(1), 15947-15947 (2017-11-23)
Stem cells therapy offers a viable alternative for treatment of bone disorders to the conventional bone grafting. However clinical therapies are still hindered by the insufficient knowledge on the conditions that maximize stem cells differentiation. Hereby, we introduce a novel
Helle Lysdahl et al.
BioResearch open access, 3(6), 278-285 (2014-12-04)
Clinical trials using bone morphogenetic protein-2 (BMP2) for bone reconstruction have shown promising results. However, the relatively high concentration needed to be effective raises concerns for efficacy and safety. The aim of this study was to investigate the osteogenic effect
Dan Huang et al.
International journal of nanomedicine, 12, 7483-7500 (2017-10-27)
Titanium (Ti) and its alloys have been widely used in clinics for years. However, their bio-inert surface challenges application in patients with compromised surgical conditions. Numerous studies were conducted to modify the surface topography and chemical composition of Ti substrates

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service