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Merck
CN

P7998

Alkaline Phosphatase Yellow

alkaline phosphatase substrate, chromogenic liquid

Synonym(s):

Alkaline phosphatase substrate solution (pNPP)

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About This Item

UNSPSC Code:
12352204
eCl@ss:
42010105
NACRES:
NA.83

Key Documents

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Product Name

Alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for ELISA, ready to use solution

Quality Level

200

form

liquid

storage temp.

2-8°C

Related Categories

General description

The product is ready to use buffered alkaline phosphatase substrate consist of p-nitrophenylphosphate (pNPP). This is the advanced substrate system contains insoluble stabilizer that enhances the stability and reactivity as well as reduces the base-line absorption. It produces a soluble end product, hence not recommended for blotting or histochemistry applications.

Application

Develops a soluble yellow reaction product that may be read at 405 nm. For endpoint assays, the reaction may be stopped with 3 N NaOH. Recommended for ELISA (microwell) procedures; not recommended for membrane applications.

Physical form

Ready to use

Preparation Note

Contains p-nitrophenyl phosphate, a substrate for alkaline phosphatase.

Disclaimer

 Stable at least one year at 2-8 °C.

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Statements

H315,H318,H373

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000457411
0000457411
0000457409
0000457409
0000389093

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Cristina A Teixeira et al.
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Laura C Jacques et al.
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Baoxin Wang et al.
Experimental and therapeutic medicine, 13(1), 178-186 (2017-01-27)
Damage to the recurrent laryngeal nerve often causes hoarseness, dyspnea, dysphagia, and sometimes asphyxia due to vocal cord paralysis which result in a reduction of quality of life. Brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) play
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Toxicologic pathology, 49(7), 1255-1268 (2021-09-09)
COVID-19 is a rapidly spreading disease, posing a huge hazard to global health. The plasmid vaccine pTK1A-TPA-SpikeA (named COVID-eVax) encodes the severe acute respiratory syndrome coronavirus 2 S protein receptor-binding domain, developed for intramuscular injection followed by electroporation (EP). The
Xin-Yu Guo et al.
The Journal of biological chemistry, 295(48), 16393-16410 (2020-09-25)
The protein folding and lipid moiety status of glycosylphosphatidylinositol-anchored proteins (GPI-APs) are monitored in the endoplasmic reticulum (ER), with calnexin playing dual roles in the maturation of GPI-APs. In the present study, we investigated the functions of calnexin in the
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比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

Related Content

Data Sheet - P7998

Instructions

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