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X114

Sigma-Aldrich

Triton™ X-114

laboratory grade

Synonym(s):

(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, Polyethylene glycol tert-octylphenyl ether

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About This Item

Linear Formula:
(C2H4O)n C14H22O, n = 7 or 8
CAS Number:
9036-19-5
Beilstein:
2315025
MDL number:
MFCD00128254
UNSPSC Code:
12161900
PubChem Substance ID:
24902129
NACRES:
NA.21

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grade

laboratory grade

Quality Level

200

description

non-ionic

mol wt

average mol wt 537

composition

t-Oct-C6H4-(OCH2CH2)xOH, x = 7-8

refractive index

n20/D 1.488 (lit.)

CMC

0.2 mM (20-25°C)

transition temp

pour point ~−9 °C
cloud point 23 °C

density

1.058 g/mL at 25 °C (lit.)

HLB

12.4

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density

1.045 g/mL at 25 °C (lit.)

density

1.045 g/mL at 25 °C (lit.)

density

0.975 g/mL at 25 °C (lit.)

density

0.917 g/mL at 25 °C (lit.)

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

bp

190 °C (lit.)

bp

190 °C (lit.)

bp

106 °C (lit.)

bp

118-119 °C (lit.)

refractive index

n20/D 1.431 (lit.)

refractive index

n20/D 1.431 (lit.)

refractive index

-

refractive index

n20/D 1.413 (lit.)

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

2-8°C

General description

Triton™ X-114 is a nonionic detergent. It is a good detergent for solubilizing membranes having reversible phase-separation property useful for the separation of intrinsic membrane proteins.[1] Solution of Triton™ X-114 is homogeneous at 0°C but phase separation into aqueous phase and a detergent phase occurs above 20°C.[2] Phase separation of proteins in solutions of Triton™ X-114 has been investigated.[1] This octylphenol ethoxylate is compatible with anionic, cationic, and other nonionic surfactants and chemically stable in most acidic & alkaline solutions.

Application

Triton™ X-114 has been used:
  • To remove endotoxin from solutions of protein using phase separation technique[3]
  • In the extraction and purification of DNA from endotoxin[4]
  • In the biphasic extraction of lipoproteins from bacterial cell wall[5][6]
  • As a non-ionic detergent with a low cloud point (23 °C) enabling protein solubilization with phase-partitioning of hydrophilic from amphiphilic proteins

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Signal Word

Danger

Hazard Statements

H302,H315,H318,H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

483.8 °F - closed cup

Flash Point(C)

251 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    Certificates of Analysis (COA)

    Lot/Batch Number
    MKCX0957
    MKCW6472
    MKCV5666
    MKCW4012
    0000279694

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    TLR2 and its co-receptors determine responses of macrophages and dendritic cells to lipoproteins of Mycobacterium tuberculosis.
    Golenbock D T, et al.
    Cellular Immunology, 258(1), 29-37 (2009)
    G Gabarrini et al.
    Journal of dental research, 97(5), 556-562 (2018-01-05)
    Porphyromonas gingivalis is one of the major oral pathogens implicated in the widespread inflammatory disorder periodontitis. Moreover, in recent years, P. gingivalis has been associated with the autoimmune disease rheumatoid arthritis. The peptidylarginine deiminase enzyme of P. gingivalis (PPAD) is
    Mycobacterium tuberculosis LprG (Rv1411c): a novel TLR-2 ligand that inhibits human macrophage class II MHC antigen processing.
    Gehring A J, et al.
    Journal of Immunology, 173(4), 2660-2668 (2004)
    Nozomu Takahashi et al.
    FEBS open bio, 7(9), 1338-1349 (2017-09-15)
    Neuroglobin (Ngb) is a heme protein expressed in the vertebrate brain. We previously engineered a chimeric Ngb protein, in which module M1 of human Ngb is replaced by that of zebrafish Ngb, and showed that the chimeric ZHHH Ngb has
    Huanhuan Li et al.
    Life sciences, 231, 116548-116548 (2019-06-11)
    Multidrug resistance is a major obstacle in chemotherapy, which is mainly caused by the overexpression of ATP-binding cassette (ABC) transporters. Breast cancer resistance protein (BCRP) is one of the ABC transporters and is strongly associated with multidrug resistance. Results of
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