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93443

Triton™ X-100 solution

BioUltra, Molecular Biology, ~10% in H2O

Synonym(s):

Triton™ X-100, 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol solution, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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About This Item

Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
9036-19-5
UNSPSC Code:
12161900
NACRES:
NA.25
PubChem Substance ID:
57653560
MDL number:
MFCD00128254
Beilstein/REAXYS Number:
2315025
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grade

Molecular Biology

Quality Segment

200

description

non-ionic

product line

BioUltra

form

liquid

mol wt

micellar avg mol wt 80,000, average mol wt 625

concentration

~10% in H2O

aggregation number

100-155

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

refractive index

n20/D 1.349±0.002

pH

6.5-8.5 (25 °C)

CMC

0.2-0.9 mM (20-25°C), 0.23 mM

mp

6 °C

transition temp

cloud point 65 °C, pour point ~7 °C

solubility

water: soluble

density

1.01 g/mL at 20 °C, 1.070 g/cm3

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

cation traces

Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤100 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Na: ≤100 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

absorption

cut-off at 310 nm

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

General description

Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton™ X-100 has been used:
  • for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometry
  • As a component of lysis buffer used for homogenization of cells
  • As a component of blocking buffer in immunofluorescence staining
  • For the preparation of cells in double fluorescence staining

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Features and Benefits

  • Suitable for Molecular Biology and Biochemical Research
  • Free from DNase, RNase, NICKase and protease
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications.
  • High purity product for research applications
  • Tested for the presence of Anionic and Cationic traces

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Danger

Storage Class

10 - Combustible liquids

flash_point_f

483.8 °F - closed cup

flash_point_c

251 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2

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