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E4274

Sigma-Aldrich

Endotoxin Removal solution

Remove lipopolysaccharides (LPS) from plasmid DNA preparations

Synonym(s):

ET removal buffer, ET removal reagent, ET removal solution, endo removal buffer, endo removal reagent, endo removal solution, endotoxin contamination removal, endotoxin removal buffer, endotoxin removal reagent, plasmid purification endo, plasmid purification endo free

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25 ML
CN¥731.92
1 L
CN¥2,617.16

CN¥731.92

Available to ship onApril 17, 2025Details

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25 ML
CN¥731.92
1 L
CN¥2,617.16

About This Item

UNSPSC Code:
12352200
NACRES:
NA.52

CN¥731.92

Available to ship onApril 17, 2025Details

Quality Level

200

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1 of 4

This Item
741949753629753610
金纳米颗粒 40 nm diameter, OD 1, stabilized suspension in 0.1 mM PBS, reactant free

753637

金纳米颗粒

金纳米颗粒 5 nm diameter, OD 1, stabilized suspension in citrate buffer

741949

金纳米颗粒

金纳米颗粒 30 nm diameter, OD 1, stabilized suspension in 0.1 mM PBS, reactant free

753629

金纳米颗粒

金纳米颗粒 20 nm diameter, OD 1, stabilized suspension in 0.1 mM PBS, reactant free

753610

金纳米颗粒

concentration

~7.2E+10 particles/mL

concentration

~5.5E+13 particles/mL

concentration

~1.8E+11 particles/mL

concentration

~6.54E+11 particles/mL

OD

1

OD

1

OD

1

OD

1

diameter

40 nm

diameter

5 nm

diameter

30 nm

diameter

20 nm

λmax

526-531 nm

λmax

510-520 nm

λmax

523-526 nm

λmax

518-522 nm

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

Endotoxin Removal solution has been used to remove lipopolysaccharide (LPS) from Baker′s yeast RNA.[1]

Biochem/physiol Actions

The reagent relies on the micellar properties of Triton X-114 to remove endotoxins from solution. When warmed above 20 °C, micelles aggregate into a separate phase that sequesters amphiphilic solutes, including lipopolysaccharides. The hydrophilic DNA remains in the aqueous phase.

Quantity

1 volume treats 5 volumes of DNA solution (≤1mg/ml)

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCR2463
    SLCR2463
    SLCM9163
    SLCH4131
    SLCF9991

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    Determination of endotoxin through an aptamer-based impedance biosensor.
    Su W
    Biosensors And Bioelectronics, 32(1), 32-36 (2012)
    M Cotten et al.
    Gene therapy, 1(4), 239-246 (1994-07-01)
    Endotoxin (lipopolysaccharide, LPS) is commonly found as a contaminant in plasmid DNA preparations. We demonstrate here that the quantities of LPS typically contaminating DNA preparations can generate a toxicity to primary cells (primary human skin fibroblasts, primary human melanoma cells)

    Articles

    Cell Culture FAQs: Bacterial Endotoxin Contamination

    FAQs on bacterial endotoxin contamination, details on endotoxin testing using the LAL assay, and tips to avoid contamination in cell cultures.

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